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Literature summary for 4.2.1.118 extracted from
- Structurally diverse dehydroshikimate dehydratase variants participate in microbial quinate catabolism (2017), Mol. Microbiol., 103, 39-54 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene asbF, sequence comparisons and phylogenetic analysis | Bacillus anthracis |
| gene quiC1, sequence comparisons and phylogenetic analysis | Pseudomonas aeruginosa |
| gene quiC1, sequence comparisons and phylogenetic analysis, plasmid-based expression of Pseudomonas putida QuiC1 in the Pseudomonas aeruginosa quiC1 knockout strain, recombinant expression of wild-type and mutant enzymes | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E356A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Pseudomonas putida |
| H168A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Pseudomonas putida |
| additional information | quiC1 transposon quiC1 gene knockout in strain PAO1, recombinant plasmid-based expression of gene quiC1 from Pseudomonas putida QuiC1 in the Pseudomonas aeruginosa quiC1 knockout strain | Pseudomonas aeruginosa |
| additional information | the isolated N-terminal domain shows about 1/3 of the wild-type enzyme activity | Pseudomonas putida |
| R207A | site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme | Pseudomonas putida |
| R210A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
| S206A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.134 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant H168A | Pseudomonas putida |  |
| 0.205 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant isolated N-terminal domain of the enzyme | Pseudomonas putida | |
| 0.331 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant wild-type enzyme | Pseudomonas putida | |
| 0.429 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant E356A | Pseudomonas putida | |
| 2.745 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant S206A | Pseudomonas putida | |
| 5.311 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant R207A | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | required | Pseudomonas putida | |
| Mg2+ | enzyme bound | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydro-shikimate | Pseudomonas aeruginosa | - |
protocatechuate + H2O | - |
? | |
| 3-dehydro-shikimate | Pseudomonas putida | - |
protocatechuate + H2O | - |
? | |
| 3-dehydro-shikimate | Bacillus anthracis | - |
protocatechuate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | Q81RQ4 | - |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas putida | A0A379KX44 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydro-shikimate | - |
Pseudomonas aeruginosa | protocatechuate + H2O | - |
? | |
| 3-dehydro-shikimate | - |
Pseudomonas putida | protocatechuate + H2O | - |
? | |
| 3-dehydro-shikimate | - |
Bacillus anthracis | protocatechuate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three-dimensional structure comparisons, superimposition of the QuiC1 N-terminal (DSD) domain with Bacillus anthracis AsbF and structure comparisons with Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), overview | Pseudomonas putida |
| More | three-dimensional structure comparisons, superimposition of the QuiC1 N-terminal (DSD) domain with Bacillus anthracis AsbF, and structure comparisons with Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), overview | Pseudomonas aeruginosa |
| More | three-dimensional structure comparisons, superimposition of the QuiC1 N-terminal (DSD) domain with Bacillus anthracis AsbF, and structure comparisons with Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), overview | Bacillus anthracis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AsbF | - |
Bacillus anthracis |
| dehydroshikimate dehydratase | - |
Pseudomonas aeruginosa |
| dehydroshikimate dehydratase | - |
Pseudomonas putida |
| dehydroshikimate dehydratase | - |
Bacillus anthracis |
| DSD | - |
Pseudomonas aeruginosa |
| DSD | - |
Pseudomonas putida |
| DSD | - |
Bacillus anthracis |
| Qui1 | - |
Pseudomonas aeruginosa |
| Qui1 | - |
Pseudomonas putida |
| quiC1 | - |
Pseudomonas aeruginosa |
| quiC1 | - |
Pseudomonas putida |
| quiC_1 | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.7 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant H168A | Pseudomonas putida | |
| 61.2 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant isolated N-terminal domain of the enzyme | Pseudomonas putida | |
| 99.1 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant R207A | Pseudomonas putida | |
| 138.2 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant E356A | Pseudomonas putida | |
| 148.1 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant S206A | Pseudomonas putida | |
| 163.6 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant wild-type enzyme | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas putida |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the QuiC1 protein is not related by sequence with previously identified DSDs from the Gram-negative genus, Acinetobacter, but instead shows limited sequence identity in its N-terminal half with fungal DSDs | Pseudomonas aeruginosa |
| evolution | the QuiC1 protein is not related by sequence with previously identified DSDs from the Gram-negative genus, Acinetobacter, but instead shows limited sequence identity in its N-terminal half with fungal DSDs | Pseudomonas putida |
| additional information | enzyme structure-function analysis, overview | Pseudomonas aeruginosa |
| additional information | superimposition of the active sites of the Pseudomonas putida QuiC1 N-terminal domain and Bacillus anthracis AsbF in complex with protocatechuate. AsbF residues occupying the same position as QuiC1 Arg207 and Arg210 are not observed. Enzyme structure-function analysis, overview | Pseudomonas putida |
| additional information | superimposition of the active sites of the Pseudomonas putida QuiC1 N-terminal domain and Bacillus anthracis AsbF in complex with protocatechuate. AsbF residues occupying the same position as QuiC1 Arg207 and Arg210 are not observed. Enzyme structure-function analysis, overview | Bacillus anthracis |
| physiological function | enzyme Qui1 is important for growth on either quinate or shikimate | Pseudomonas aeruginosa |
| physiological function | enzyme Qui1 is important for growth on either quinate or shikimate | Pseudomonas putida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.7 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant R207A | Pseudomonas putida | |
| 53.9 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant S206A | Pseudomonas putida | |
| 57.5 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant H168A | Pseudomonas putida | |
| 298.5 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant isolated N-terminal domain of the enzyme | Pseudomonas putida | |
| 322.2 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant mutant E356A | Pseudomonas putida | |
| 494.3 | - |
3-dehydro-shikimate | pH 7.5, temperature not specified in the publication, recombinant wild-type enzyme | Pseudomonas putida | |
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