Cloned (Comment) | Organism |
---|---|
gene acnD, the sequence of prp genes is prpR-prpB-prpC-acnD-prpF, functional recombinant coexpression of the genes acnD and prpF from Shewanella oneidensis in Salmonella enterica strain DELTAprpD, recombinant expression of His-tagged enzyme from gene acnD in Escherichia coli strain BL21(DE3) | Shewanella oneidensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-2-methylcitrate | Shewanella oneidensis | - |
2-methyl-trans-aconitate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella oneidensis | Q8EJW3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Shewanella oneidensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-2-methylcitrate | - |
Shewanella oneidensis | 2-methyl-trans-aconitate + H2O | - |
? | |
additional information | AcnD has aconitase-like activity | Shewanella oneidensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcnD | - |
Shewanella oneidensis |
SO_0343 | - |
Shewanella oneidensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | assay at | Shewanella oneidensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Shewanella oneidensis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. The 2-methylcitric acid cycle is a common route of propionate catabolism in microorganisms | Shewanella oneidensis |
metabolism | the enzyme is involved in the 2-methylcitric acid cycle, overview | Shewanella oneidensis |
physiological function | the two genes acnD and prpF, encoding 2-methylcitrate dehydratase (AcnD, EC 4.2.1.117) and 2-methyl-aconitate isomerase (SoPrpF, EC 5.3.3.-) from Shewanella oneidensis together can replace gene prpD from Salmonella enterica, which encodes 2-methylcitrate dehydratase, PrpD /EC 4.2.1.79. SoAcnD and SePrpD synthesize different methylaconitate isomers. The SoPrpF protein isomerizes the product of the AcnD reaction into the PrpD product (2-MCA), known substrate of the housekeeping aconitase. It is likely that 4-methylaconitate is the product of the AcnD enzyme. PrpF (SoPrpF) protein isomerizes the product of the SoAcnD dehydratase (putatively 4-methyl-cis-aconitate (4-MCA)) to 2-methyl-cis-aconitate (2-MCA). Thus,the role of SoPrpF in the 2-methylcitrate cycle (2-MCC) is to change the stereochemistry of the SoAcnD reaction product. AcnD and PrpF activities are necessary and sufficient to compensate for the lack of PrpD during growth with propionate of the strain of Salmonella enterica. 2-Methylcitrate is dehydrated by AcnD to 4-methyl-cis-aconitate, which is then isomerized to 2-methyl-cis-aconitate by PrpF, and rehydrated to 2-methylisocitrate by aconitases A and B | Shewanella oneidensis |