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Literature summary for 4.2.1.117 extracted from

  • Rocco, C.J.; Wetterhorn, K.M.; Garvey, G.S.; Rayment, I.; Escalante-Semerena, J.C.
    The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle (2017), PLoS ONE, 12, e0188130 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene acnD, the sequence of prp genes is prpR-prpB-prpC-acnD-prpF, functional recombinant coexpression of the genes acnD and prpF from Shewanella oneidensis in Salmonella enterica strain DELTAprpD, recombinant expression of His-tagged enzyme from gene acnD in Escherichia coli strain BL21(DE3) Shewanella oneidensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S,3S)-2-methylcitrate Shewanella oneidensis
-
2-methyl-trans-aconitate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Shewanella oneidensis Q8EJW3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Shewanella oneidensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-2-methylcitrate
-
Shewanella oneidensis 2-methyl-trans-aconitate + H2O
-
?
additional information AcnD has aconitase-like activity Shewanella oneidensis ?
-
?

Synonyms

Synonyms Comment Organism
AcnD
-
Shewanella oneidensis
SO_0343
-
Shewanella oneidensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37 assay at Shewanella oneidensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Shewanella oneidensis

General Information

General Information Comment Organism
metabolism the enzyme is involved in catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. The 2-methylcitric acid cycle is a common route of propionate catabolism in microorganisms Shewanella oneidensis
metabolism the enzyme is involved in the 2-methylcitric acid cycle, overview Shewanella oneidensis
physiological function the two genes acnD and prpF, encoding 2-methylcitrate dehydratase (AcnD, EC 4.2.1.117) and 2-methyl-aconitate isomerase (SoPrpF, EC 5.3.3.-) from Shewanella oneidensis together can replace gene prpD from Salmonella enterica, which encodes 2-methylcitrate dehydratase, PrpD /EC 4.2.1.79. SoAcnD and SePrpD synthesize different methylaconitate isomers. The SoPrpF protein isomerizes the product of the AcnD reaction into the PrpD product (2-MCA), known substrate of the housekeeping aconitase. It is likely that 4-methylaconitate is the product of the AcnD enzyme. PrpF (SoPrpF) protein isomerizes the product of the SoAcnD dehydratase (putatively 4-methyl-cis-aconitate (4-MCA)) to 2-methyl-cis-aconitate (2-MCA). Thus,the role of SoPrpF in the 2-methylcitrate cycle (2-MCC) is to change the stereochemistry of the SoAcnD reaction product. AcnD and PrpF activities are necessary and sufficient to compensate for the lack of PrpD during growth with propionate of the strain of Salmonella enterica. 2-Methylcitrate is dehydrated by AcnD to 4-methyl-cis-aconitate, which is then isomerized to 2-methyl-cis-aconitate by PrpF, and rehydrated to 2-methylisocitrate by aconitases A and B Shewanella oneidensis