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Literature summary for 4.2.1.116 extracted from

  • Luo, H.; Zhou, D.; Liu, X.; Nie, Z.; Quiroga-Sanchez, D.; Chang, Y.
    Production of 3-hydroxypropionic acid via the propionyl-CoA pathway using recombinant Escherichia coli strains (2016), PLoS ONE, 11, e0156286 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.116

Cloned(Commentary)

Cloned (Comment) Organism
gene hpcd, coexpression of 3-hydroxypropionyl-CoA dehydratase (HPCD) with propionyl-CoA dehydrogenase (PACD) encoded by gene pacd from Candida rugosa and propionate CoA-transferase (PCT) encoded by gene pct from Megasphaera elsdenii in Escherichia coli strain BL21(DE3) under control of the T7 promoter Chloroflexus aurantiacus

Protein Variants

Protein Variants Comment Organism
additional information production of the commercially promising platform chemical 3-hydroxypropionic acid (3-HP) via the propionyl-CoA pathway in genetically engineered Escherichia coli strain BL21(DE3). Propionate CoA-transferase from Megasphaera elsdenii and 3-hydroxypropionyl-CoA dehydratase (HPCD) from Chloroflexus aurantiacus are expressed along with propionyl-CoA dehydrogenase (PACD) from Candida rugosa, the 3-hydroxypropanoate titer of the resulting Escherichia coli Ec-PPH strain is improved by 6fold. When cultured at 30°C with 1% glucose in addition to propionate, 3-hydroxypropanoate production by Ec-PPH increases 2fold and 12fold compared to the cultivation at 37°C (4.23 mM) or without glucose (0.68 mM), respectively. Deletion of the ygfH gene encoding propionyl-CoA: succinate CoA-transferase from Ec-PPH (resulting in the strain Ec-DELTAY-PPH) leads to increase of 3-hydroxypropanoate production in shake flask experiments (15.04 mM), whereas the strain Ec-DELTAY-PPH with deletion of the prpC gene (encoding methylcitrate synthase in the methylcitrate cycle) produces 17.76 mM of 3-HP. The strain Ec-DELTAY-DELTAP-PPH with both ygfH and prpC genes deleted produces 24.14 mM of 3-HP, thus showing an 18fold increase in the 3-hydroxypropanoate titer in compare to the strain Ec-P. Disruption of the competing metabolic pathways. Established transgenic metabolic pathway, method, overview Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropanoyl-CoA Chloroflexus aurantiacus
-
 acryloyl-CoA + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropanoyl-CoA
-
 Chloroflexus aurantiacus acryloyl-CoA + H2O
-
 r

Synonyms

Synonyms Comment Organism
3-hydroxypropionyl-CoA dehydratase
-
 Chloroflexus aurantiacus
HPCD
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo Chloroflexus aurantiacus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 37 recombinant PACD activity in vivo is the highest at 30°C, it decreased to 30% activity when incubated for 2 h at 37°C Chloroflexus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 37 recombinant PACD activity in vivo is the highest at 30°C, it decreased to 30% activity when incubated for 2 h at 37°C Chloroflexus aurantiacus

General Information

General Information Comment Organism
metabolism propionyl-CoA is a key precursor for 3-hydroxypropanoate formation Chloroflexus aurantiacus