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Literature summary for 4.2.1.114 extracted from

  • Lee, E.H.; Lee, K.; Hwang, K.Y.
    Structural characterization and comparison of the large subunits of IPM isomerase and homoaconitase from Methanococcus jannaschii (2014), Acta Crystallogr. Sect. D, 70, 922-931.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Star cells Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion method and hanging-drop vapour diffusion at 20°C, structures of the large subunit of homoaconise is reported at 2.5 A resolution Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur cluster the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58409 large subunit of homoaconitase
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Methanocaldococcus jannaschii DSM 2661 Q58409 large subunit of homoaconitase
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Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
HacA
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Methanocaldococcus jannaschii
Homoaconitase
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Methanocaldococcus jannaschii
MJ1003 gene name, large subunit of homoaconitase Methanocaldococcus jannaschii