Literature summary for 4.2.1.113 extracted from

Klenchin, V.A.; Taylor Ringia, E.A.; Gerlt, J.A.; Rayment, I.
Evolution of enzymatic activity in the enolase superfamily: structural and mutagenic studies of the mechanism of the reaction catalyzed by o-succinylbenzoate synthase from Escherichia coli (2003), Biochemistry, 42, 14427-14433.

Search for more literature for 4.2.1.113

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli of several recombinant mutant enzymes	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
co-crystallization of the K133R mutant with 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K133A	inactive mutant	Escherichia coli
K133R	inactive mutant	Escherichia coli
K133S	inactive mutant	Escherichia coli
K235A	inactive mutant	Escherichia coli
K235R	inactive mutant	Escherichia coli
K235S	inactive mutant	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37740	-	mass spectrometry	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid	Escherichia coli	-	4-(2-carboxyphenyl)-4-oxobutyrate + H2O	-	ir
additional information	Escherichia coli	involved in the menaquinone biosynthetic pathway	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P29208	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid	-	Escherichia coli	4-(2-carboxyphenyl)-4-oxobutyrate + H2O	-	?
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid	-	Escherichia coli	4-(2-carboxyphenyl)-4-oxobutyrate + H2O	-	ir
additional information	involved in the menaquinone biosynthetic pathway	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
OSBS	-	Escherichia coli

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Release 2023.1 (January 2023)