Cloned (Comment) | Organism |
---|---|
expression of a recombinant enzyme in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystallization of the apoenzyme and a complex with Mg2+ and 4-(2-carboxyphenyl)-4-oxobutyrate | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for activity | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35760 | - |
mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid | Escherichia coli | - |
4-(2-carboxyphenyl)-4-oxobutyrate + H2O | - |
ir | |
additional information | Escherichia coli | involved in the menaquinone biosynthetic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P29208 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of a recombinant His-tagged enzyme by affinity chromatography followed by tag cleavage and anion-exchange chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid | - |
Escherichia coli | 4-(2-carboxyphenyl)-4-oxobutyrate + H2O | - |
? | |
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid | - |
Escherichia coli | 4-(2-carboxyphenyl)-4-oxobutyrate + H2O | - |
ir | |
additional information | involved in the menaquinone biosynthetic pathway | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OSBS | - |
Escherichia coli |