Crystallization (Comment) | Organism |
---|---|
purified enzyme, the mother liquor contains plus 15% (v/v) 2-methyl-2,5-pentanediol, X-ray diffraction structure determination and analysis at 1.26-1.95 A resolution, modeling | Syntrophotalea acetylenica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | non-redox-active tungsten/[4Fe-4S] enzyme, a cubane-type [4Fe:4S] cluster, structure analysis, overview | Syntrophotalea acetylenica | |
Tungsten | a non-redox-active tungsten/[4Fe-4S] enzyme, contains two molybdopterin guanine dinucleotide cofactors, MGD, designated P and Q, structure analysis, bis-molybdopterin guanine dinucleotide-ligated tungsten atom, overview, the tungsten center binds a water molecule that is activated by an adjacent aspartate residue, enabling to attack acetylene bound in a distinct, hydrophobic pocket, this mechanism requires a strong shift of pKa of the aspartate, caused by nearby low-potential [4Fe:4S] cluster, overview | Syntrophotalea acetylenica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene + H2O | Syntrophotalea acetylenica | - |
acetaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Syntrophotalea acetylenica | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetaldehyde = acetylene + H2O | active site channel structure and detailed catalytic mechanism | Syntrophotalea acetylenica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strictly anaerobic growth conditions | Syntrophotalea acetylenica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene + H2O | - |
Syntrophotalea acetylenica | acetaldehyde | - |
? | |
acetylene + H2O | a non-redox reaction | Syntrophotalea acetylenica | acetaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | four domain structure with active site access pathway, active site architecture, overview | Syntrophotalea acetylenica |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme structurally belongs to the dimethyl sulfoxide reductase family of enzymes | Syntrophotalea acetylenica |