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Literature summary for 4.2.1.112 extracted from

  • Rosner, B.M.; Rainey, F.A.; Kroppenstedt, R.M.; Schink, B.
    Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes (1997), FEMS Microbiol. Lett., 148, 175-180.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme does not require reductive additives Rhodococcus ruber
additional information the enzyme does not require reductive additives Gordonia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum dependent on, enzyme expression also requires molybdenum Rhodococcus ruber
Molybdenum dependent on, enzyme expression also requires molybdenum Rhodococcus opacus
Molybdenum dependent on, enzyme expression also requires molybdenum Gordonia sp.
additional information the enzyme does not require reductive additives Rhodococcus ruber
additional information the enzyme does not require reductive additives Gordonia sp.
Ti(III)citrate a strong reductant is absolutely required for activity Rhodococcus opacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylene + H2O Rhodococcus ruber
-
acetaldehyde
-
?
acetylene + H2O Rhodococcus opacus
-
acetaldehyde
-
?
acetylene + H2O Gordonia sp.
-
acetaldehyde
-
?
acetylene + H2O Rhodococcus ruber aerobic
-
acetaldehyde
-
?
acetylene + H2O Gordonia sp. aerobic
-
acetaldehyde
-
?

Organism

Organism UniProt Comment Textmining
Gordonia sp.
-
isolated from soil, aerobic strain
-
Gordonia sp. aerobic
-
isolated from soil, aerobic strain
-
Rhodococcus opacus
-
two aerobic isolates from soil, DSM 44186 and 44188
-
Rhodococcus ruber
-
isolated from soil, aerobic strain
-
Rhodococcus ruber aerobic
-
isolated from soil, aerobic strain
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetylene-grown cell
-
Rhodococcus ruber
-
culture condition:acetylene-grown cell
-
Rhodococcus opacus
-
culture condition:acetylene-grown cell
-
Gordonia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhodococcus ruber
additional information
-
-
Rhodococcus opacus
additional information
-
-
Gordonia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylene + H2O
-
Rhodococcus ruber acetaldehyde
-
?
acetylene + H2O
-
Rhodococcus opacus acetaldehyde
-
?
acetylene + H2O
-
Gordonia sp. acetaldehyde
-
?
acetylene + H2O
-
Rhodococcus ruber aerobic acetaldehyde
-
?
acetylene + H2O
-
Gordonia sp. aerobic acetaldehyde
-
?