Data extracted from this reference:
Activating Compound
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Metals/Ions
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus ruber
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus opacus
Molybdenum
dependent on, enzyme expression also requires molybdenum
Gordonia sp.
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Ti(III)citrate
a strong reductant is absolutely required for activity
Rhodococcus opacus
Natural Substrates/ Products (Substrates)
acetylene + H2O
Rhodococcus ruber
acetaldehyde
?
acetylene + H2O
Rhodococcus opacus
acetaldehyde
?
acetylene + H2O
Gordonia sp.
acetaldehyde
?
acetylene + H2O
Rhodococcus ruber aerobic
acetaldehyde
?
acetylene + H2O
Gordonia sp. aerobic
acetaldehyde
?
Organism
Gordonia sp.
isolated from soil, aerobic strain
Gordonia sp. aerobic
isolated from soil, aerobic strain
Rhodococcus opacus
two aerobic isolates from soil, DSM 44186 and 44188
Rhodococcus ruber
isolated from soil, aerobic strain
Rhodococcus ruber aerobic
isolated from soil, aerobic strain
Source Tissue
culture condition:acetylene-grown cell
Rhodococcus ruber
culture condition:acetylene-grown cell
Rhodococcus opacus
culture condition:acetylene-grown cell
Gordonia sp.
Specific Activity [micromol/min/mg]
additional information
Rhodococcus ruber
additional information
Rhodococcus opacus
additional information
Gordonia sp.
Substrates and Products (Substrate)
acetylene + H2O
668651
Rhodococcus ruber
acetaldehyde
?
acetylene + H2O
668651
Rhodococcus opacus
acetaldehyde
?
acetylene + H2O
668651
Gordonia sp.
acetaldehyde
?
acetylene + H2O
668651
Rhodococcus ruber aerobic
acetaldehyde
?
acetylene + H2O
668651
Gordonia sp. aerobic
acetaldehyde
?
Activating Compound (protein specific)
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Metals/Ions (protein specific)
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus ruber
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus opacus
Molybdenum
dependent on, enzyme expression also requires molybdenum
Gordonia sp.
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Ti(III)citrate
a strong reductant is absolutely required for activity
Rhodococcus opacus
Natural Substrates/ Products (Substrates) (protein specific)
acetylene + H2O
Rhodococcus ruber
acetaldehyde
?
acetylene + H2O
Rhodococcus opacus
acetaldehyde
?
acetylene + H2O
Gordonia sp.
acetaldehyde
?
acetylene + H2O
Rhodococcus ruber aerobic
acetaldehyde
?
acetylene + H2O
Gordonia sp. aerobic
acetaldehyde
?
Source Tissue (protein specific)
culture condition:acetylene-grown cell
Rhodococcus ruber
culture condition:acetylene-grown cell
Rhodococcus opacus
culture condition:acetylene-grown cell
Gordonia sp.
Specific Activity [micromol/min/mg] (protein specific)
additional information
Rhodococcus ruber
additional information
Rhodococcus opacus
additional information
Gordonia sp.
Substrates and Products (Substrate) (protein specific)
acetylene + H2O
668651
Rhodococcus ruber
acetaldehyde
?
acetylene + H2O
668651
Rhodococcus opacus
acetaldehyde
?
acetylene + H2O
668651
Gordonia sp.
acetaldehyde
?
acetylene + H2O
668651
Rhodococcus ruber aerobic
acetaldehyde
?
acetylene + H2O
668651
Gordonia sp. aerobic
acetaldehyde
?
Other publictions for EC 4.2.1.112
748243
Kroneck
Acetylene hydratase a non-re ...
Rhodococcus opacus, Rhodococcus ruber, Rhodobacter capsulatus, Norcadia rhodochorous, Rhodococcus sp. A1, Gordona alkanivorans, Pelobacter acetylenicus
J. Biol. Inorg. Chem.
21
29-38
2016
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-
-
1
5
-
2
-
1
19
-
7
-
7
-
-
-
7
-
2
-
-
14
1
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
14
1
5
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2
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1
19
-
7
-
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2
-
-
14
1
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9
9
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-
748431
Boll
Structure and function of the ...
Pelobacter acetylenicus
J. Mol. Microbiol. Biotechnol.
26
119-137
2016
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1
5
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4
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1
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2
2
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3
1
5
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1
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1
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2
2
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2
2
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730116
Liao
Convergence in the QM-only and ...
Escherichia coli
J. Comput. Chem.
34
2389-2397
2013
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1
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4
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1
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1
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1
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4
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2
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1
1
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-
713603
Liao
-
Theoretical study of the chemo ...
Pelobacter acetylenicus
ACS Catal.
1
937-944
2011
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1
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1
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1
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1
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2
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1
1
-
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-
715374
Tenbrink
Exploring the active site of t ...
Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246
J. Bacteriol.
193
1229-1236
2011
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1
1
4
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1
1
2
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2
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7
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1
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2
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4
1
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1
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1
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1
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1
1
1
4
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1
1
2
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2
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2
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4
1
1
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1
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-
703363
Vincent
A DFT study of the possible ro ...
Pelobacter acetylenicus
Dalton Trans.
39
3816-3822
2010
-
-
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1
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1
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1
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1
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1
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1
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1
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716769
Liao
Mechanism of tungsten-dependen ...
Pelobacter acetylenicus
Proc. Natl. Acad. Sci. USA
107
22523-22527
2010
-
-
-
-
-
-
-
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-
1
-
1
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1
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1
-
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1
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1
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1
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1
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-
706054
Antony
-
Theoretical studies of models ...
Pelobacter acetylenicus
Organometallics
28
4938-4944
2009
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
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670710
Seiffert
Structure of the non-redox-act ...
Pelobacter acetylenicus
Proc. Natl. Acad. Sci. USA
104
3073-3077
2007
-
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-
1
-
-
-
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-
2
-
1
-
1
-
-
-
1
-
1
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2
1
1
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-
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-
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-
1
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2
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1
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1
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1
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667090
Einsle
Crystallization and preliminar ...
Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246
Acta Crystallogr. Sect. F
F61
299-301
2005
2
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1
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1
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2
1
2
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7
1
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1
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1
1
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4
1
1
1
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1
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2
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1
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1
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2
1
2
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1
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1
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1
1
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4
1
1
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1
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-
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-
668453
Meckenstock
Acetylene hydratase of Pelobac ...
Pelobacter acetylenicus
Eur. J. Biochem.
264
176-182
1999
1
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1
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3
2
1
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5
1
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1
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1
2
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1
1
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1
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1
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1
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1
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1
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1
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3
2
1
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1
-
1
-
1
2
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1
1
1
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-
1
-
-
-
-
-
-
-
-
-
668651
Rosner
Acetylene degradation by new i ...
Rhodococcus opacus, Rhodococcus ruber, Gordonia sp., Rhodococcus ruber aerobic, Gordonia sp. aerobic
FEMS Microbiol. Lett.
148
175-180
1997
2
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6
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5
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14
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3
3
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5
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2
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6
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5
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3
3
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5
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669039
Yadav
-
A functional mimic of the new ...
Pelobacter acetylenicus
J. Am. Chem. Soc.
119
4315-4316
1997
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3
1
1
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1
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1
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2
1
1
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1
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1
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3
1
1
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2
1
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-
669072
Rosner
Purification and characterizat ...
Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246
J. Bacteriol.
177
5767-5772
1995
3
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6
2
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3
2
2
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10
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1
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3
1
1
4
1
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1
1
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1
1
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1
-
3
-
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6
-
2
-
3
2
2
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1
-
3
1
1
4
1
1
1
-
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1
1
-
1
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-
-
-