BRENDA - Enzyme Database
show all sequences of 4.2.1.112

Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes

Rosner, B.M.; Rainey, F.A.; Kroppenstedt, R.M.; Schink, B.; FEMS Microbiol. Lett. 148, 175-180 (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus ruber
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus opacus
Molybdenum
dependent on, enzyme expression also requires molybdenum
Gordonia sp.
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Ti(III)citrate
a strong reductant is absolutely required for activity
Rhodococcus opacus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetylene + H2O
Rhodococcus ruber
-
acetaldehyde
-
-
?
acetylene + H2O
Rhodococcus opacus
-
acetaldehyde
-
-
?
acetylene + H2O
Gordonia sp.
-
acetaldehyde
-
-
?
acetylene + H2O
Rhodococcus ruber aerobic
-
acetaldehyde
-
-
?
acetylene + H2O
Gordonia sp. aerobic
-
acetaldehyde
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Gordonia sp.
-
isolated from soil, aerobic strain
-
Gordonia sp. aerobic
-
isolated from soil, aerobic strain
-
Rhodococcus opacus
-
two aerobic isolates from soil, DSM 44186 and 44188
-
Rhodococcus ruber
-
isolated from soil, aerobic strain
-
Rhodococcus ruber aerobic
-
isolated from soil, aerobic strain
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:acetylene-grown cell
-
Rhodococcus ruber
-
culture condition:acetylene-grown cell
-
Rhodococcus opacus
-
culture condition:acetylene-grown cell
-
Gordonia sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rhodococcus ruber
additional information
-
-
Rhodococcus opacus
additional information
-
-
Gordonia sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetylene + H2O
-
668651
Rhodococcus ruber
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Rhodococcus opacus
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Gordonia sp.
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Rhodococcus ruber aerobic
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Gordonia sp. aerobic
acetaldehyde
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus ruber
Molybdenum
dependent on, enzyme expression also requires molybdenum
Rhodococcus opacus
Molybdenum
dependent on, enzyme expression also requires molybdenum
Gordonia sp.
additional information
the enzyme does not require reductive additives
Rhodococcus ruber
additional information
the enzyme does not require reductive additives
Gordonia sp.
Ti(III)citrate
a strong reductant is absolutely required for activity
Rhodococcus opacus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetylene + H2O
Rhodococcus ruber
-
acetaldehyde
-
-
?
acetylene + H2O
Rhodococcus opacus
-
acetaldehyde
-
-
?
acetylene + H2O
Gordonia sp.
-
acetaldehyde
-
-
?
acetylene + H2O
Rhodococcus ruber aerobic
-
acetaldehyde
-
-
?
acetylene + H2O
Gordonia sp. aerobic
-
acetaldehyde
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:acetylene-grown cell
-
Rhodococcus ruber
-
culture condition:acetylene-grown cell
-
Rhodococcus opacus
-
culture condition:acetylene-grown cell
-
Gordonia sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rhodococcus ruber
additional information
-
-
Rhodococcus opacus
additional information
-
-
Gordonia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetylene + H2O
-
668651
Rhodococcus ruber
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Rhodococcus opacus
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Gordonia sp.
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Rhodococcus ruber aerobic
acetaldehyde
-
-
-
?
acetylene + H2O
-
668651
Gordonia sp. aerobic
acetaldehyde
-
-
-
?
Other publictions for EC 4.2.1.112
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748243
Kroneck
Acetylene hydratase a non-re ...
Rhodococcus opacus, Rhodococcus ruber, Rhodobacter capsulatus, Norcadia rhodochorous, Rhodococcus sp. A1, Gordona alkanivorans, Pelobacter acetylenicus
J. Biol. Inorg. Chem.
21
29-38
2016
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-
1
5
-
2
-
1
19
-
7
-
7
-
-
-
7
-
2
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-
14
1
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
14
1
5
-
-
2
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-
1
19
-
7
-
-
-
-
-
2
-
-
14
1
-
-
-
-
-
-
-
-
-
9
9
-
-
-
748431
Boll
Structure and function of the ...
Pelobacter acetylenicus
J. Mol. Microbiol. Biotechnol.
26
119-137
2016
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-
-
1
5
-
2
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1
4
-
1
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2
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1
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2
2
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3
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3
1
5
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2
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1
4
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1
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2
2
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2
2
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730116
Liao
Convergence in the QM-only and ...
Escherichia coli
J. Comput. Chem.
34
2389-2397
2013
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1
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4
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1
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1
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1
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3
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1
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1
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1
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2
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1
1
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713603
Liao
-
Theoretical study of the chemo ...
Pelobacter acetylenicus
ACS Catal.
1
937-944
2011
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1
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1
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1
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1
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1
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1
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-
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2
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-
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-
-
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-
1
1
-
-
-
715374
Tenbrink
Exploring the active site of t ...
Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246
J. Bacteriol.
193
1229-1236
2011
-
-
1
1
4
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-
1
1
2
-
2
-
7
-
-
-
1
-
-
2
-
4
1
-
1
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-
1
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-
1
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-
1
1
1
4
-
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1
1
2
-
2
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2
-
4
1
1
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-
1
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-
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-
-
-
703363
Vincent
A DFT study of the possible ro ...
Pelobacter acetylenicus
Dalton Trans.
39
3816-3822
2010
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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716769
Liao
Mechanism of tungsten-dependen ...
Pelobacter acetylenicus
Proc. Natl. Acad. Sci. USA
107
22523-22527
2010
-
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-
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1
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1
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1
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1
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1
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1
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1
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1
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706054
Antony
-
Theoretical studies of models ...
Pelobacter acetylenicus
Organometallics
28
4938-4944
2009
-
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-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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670710
Seiffert
Structure of the non-redox-act ...
Pelobacter acetylenicus
Proc. Natl. Acad. Sci. USA
104
3073-3077
2007
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1
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2
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1
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1
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1
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1
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2
1
1
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1
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2
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1
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1
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2
1
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-
667090
Einsle
Crystallization and preliminar ...
Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246
Acta Crystallogr. Sect. F
F61
299-301
2005
2
-
-
1
-
-
-
1
-
2
1
2
-
7
1
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1
-
-
1
1
-
4
1
1
1
-
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1
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2
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1
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1
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2
1
2
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1
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1
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1
1
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4
1
1
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1
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668453
Meckenstock
Acetylene hydratase of Pelobac ...
Pelobacter acetylenicus
Eur. J. Biochem.
264
176-182
1999
1
-
-
-
-
-
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1
-
3
2
1
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5
1
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1
-
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1
2
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1
1
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1
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1
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1
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1
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1
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1
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3
2
1
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1
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1
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1
2
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1
1
1
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1
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-
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-
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-
-
668651
Rosner
Acetylene degradation by new i ...
Rhodococcus opacus, Rhodococcus ruber, Gordonia sp., Rhodococcus ruber aerobic, Gordonia sp. aerobic
FEMS Microbiol. Lett.
148
175-180
1997
2
-
-
-
-
-
-
-
-
6
-
5
-
14
-
-
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-
3
3
-
5
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2
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6
-
5
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3
3
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5
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669039
Yadav
-
A functional mimic of the new ...
Pelobacter acetylenicus
J. Am. Chem. Soc.
119
4315-4316
1997
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3
1
1
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1
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1
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2
1
1
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1
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1
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3
1
1
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2
1
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669072
Rosner
Purification and characterizat ...
Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246
J. Bacteriol.
177
5767-5772
1995
3
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6
2
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3
2
2
-
10
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1
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3
1
1
4
1
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1
1
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1
1
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1
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3
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6
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2
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3
2
2
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1
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3
1
1
4
1
1
1
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1
1
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1
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