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Literature summary for 4.2.1.112 extracted from

  • Einsle, O.; Niessen, H.; Abt, D.J.; Seiffert, G.; Schink, B.; Huber, R.; Messerschmidt, A.; Kroneck, P.M.H.
    Crystallization and preliminary x-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus (2005), Acta Crystallogr. Sect. F, F61, 299-301.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.112

Activating Compound

Activating Compound Comment Organism Structure
Dithionite a strong reductant is required for activity Syntrophotalea acetylenica
Ti(III)citrate a strong reductant is required for activity Syntrophotalea acetylenica

Crystallization (Commentary)

Crystallization (Comment) Organism
purified native enzyme, sitting drop vapour diffusion method in a 95%N2/5%H2 atmosphere, 10 mg/ml protein in 5 mM HEPES-NaOH, pH 7.5, and 3 mM dithionite or Ti(III)citrate, mixing with an equal volume of 0.002 ml of precipitant solution equilibrated against 0.3 ml of reservoir, 20°C, 3 weeks, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement Syntrophotalea acetylenica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme activity depends on the redox status, overview Syntrophotalea acetylenica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on, the enzyme is a tungsten/iron-sulfur protein with [4Fe-4S], N-terminal binding cluster Cys-Xaa-Cys-Xaa-Cys Syntrophotalea acetylenica
Tungsten dependent on, the enzyme is a tungsten/iron-sulfur protein Syntrophotalea acetylenica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 mass spectrometry Syntrophotalea acetylenica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylene + H2O Syntrophotalea acetylenica
-
 acetaldehyde
-
 ?
acetylene + H2O Syntrophotalea acetylenica WoAcy1 / DSM 3246
-
 acetaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Syntrophotalea acetylenica
-
-
-
Syntrophotalea acetylenica WoAcy1 / DSM 3246
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is oxygen-sensitive Syntrophotalea acetylenica

Purification (Commentary)

Purification (Comment) Organism
native enzyme 29.7fold by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration to homogeneity Syntrophotalea acetylenica

Source Tissue

Source Tissue Comment Organism Textmining
additional information the organism is grown anaerobically in tungstate-supplemented fresh water medium Syntrophotalea acetylenica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.7
-
 purified native enzyme Syntrophotalea acetylenica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylene + H2O
-
 Syntrophotalea acetylenica acetaldehyde
-
 ?
acetylene + H2O
-
 Syntrophotalea acetylenica acetaldehyde the subsequent disproportionation of acetaldehyde yields acetate and ethanol ?
acetylene + H2O
-
 Syntrophotalea acetylenica WoAcy1 / DSM 3246 acetaldehyde
-
 ?
acetylene + H2O
-
 Syntrophotalea acetylenica WoAcy1 / DSM 3246 acetaldehyde the subsequent disproportionation of acetaldehyde yields acetate and ethanol ?

Subunits

Subunits Comment Organism
monomer 1 * 85000, MALDI mass spectrometry Syntrophotalea acetylenica

Synonyms

Synonyms Comment Organism
tungsten-dependent acetylene hydratase
-
 Syntrophotalea acetylenica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Syntrophotalea acetylenica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Syntrophotalea acetylenica