BRENDA - Reference to 4.2.1.112; Id = 669072

Dithionite

a strong reductant is required for activity

Pelobacter acetylenicus

additional information

no activation by tungstate at 5 mM

Pelobacter acetylenicus

Ti(III)citrate

a strong reductant is required for activity

Pelobacter acetylenicus

CO

90% inhibition at 0.8 mM

Pelobacter acetylenicus

HgCl2

reduces enzyme activity by 40% at 0.01 mM, 80% at 0.1 mM, and 98% at 0.2 mM

Pelobacter acetylenicus

KCN

20% inhibition at 1 mM, 40% inhibition at 5-10 mM

Pelobacter acetylenicus

molybdate

-

Pelobacter acetylenicus

additional information

no inhibition by citrate, ascorbate, tiron, ferrocene, and EDTA, no inhibition by ethylene at 8 mM

Pelobacter acetylenicus

nitric oxide

complete inhibition at 3 mM

Pelobacter acetylenicus

additional information

-

additional information

the enzyme activity depends on the redox status, overview

Pelobacter acetylenicus

0.014

-

Acetylene

pH 7.0, 30°C

Pelobacter acetylenicus

Iron-sulfur cluster

dependent on, the enzyme is a tungsten/iron-sulfur protein, 4.8 mol of iron per mol of enzyme and 3.9 mol od acid-labile sulfur per mol of enzyme

Pelobacter acetylenicus

additional information

no molybdenum detectable

Pelobacter acetylenicus

Tungsten

dependent on, the enzyme is a tungsten/iron-sulfur protein, 0.4 mol of tungsten per mol of enzyme

Pelobacter acetylenicus

60000

-

gel filtration

Pelobacter acetylenicus

73000

-

1 * 73000, SDS-PAGE

Pelobacter acetylenicus

acetylene + H2O

Pelobacter acetylenicus

-

acetaldehyde

-

?

acetylene + H2O

Pelobacter acetylenicus WoAcy1 / DSM 3246

-

acetaldehyde

-

?

Pelobacter acetylenicus

-

Pelobacter acetylenicus WoAcy1 / DSM 3246

-

under air at room temperature, native enzyme 240-fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and a second anion exchange chromatography step to homogeneity

Pelobacter acetylenicus

culture condition:acetylene-grown cell

-

Pelobacter acetylenicus

-

culture condition:molybdate-grown cell

-

Pelobacter acetylenicus

-

culture condition:tungstate-grown cell

-

Pelobacter acetylenicus

-

69.2

-

purified enzyme

Pelobacter acetylenicus

enzyme activity is stable even after prolonged storage of the cell extract or of the purified protein under air

Pelobacter acetylenicus

acetylene + H2O

-

669072

Pelobacter acetylenicus

acetaldehyde

-

?

acetylene + H2O

-

669072

Pelobacter acetylenicus WoAcy1 / DSM 3246

acetaldehyde

-

?

additional information

no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity

669072

Pelobacter acetylenicus

?

-

?

additional information

no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity

669072

Pelobacter acetylenicus WoAcy1 / DSM 3246

?

-

?

monomer

1 * 73000, SDS-PAGE

Pelobacter acetylenicus

50

-

Pelobacter acetylenicus

22

80

-

Pelobacter acetylenicus

6

6.5

-

Pelobacter acetylenicus

4

9

-

Pelobacter acetylenicus

chromatofocusing

-

4.3

Dithionite

a strong reductant is required for activity

Pelobacter acetylenicus

additional information

no activation by tungstate at 5 mM

Pelobacter acetylenicus

Ti(III)citrate

a strong reductant is required for activity

Pelobacter acetylenicus

CO

90% inhibition at 0.8 mM

Pelobacter acetylenicus

HgCl2

reduces enzyme activity by 40% at 0.01 mM, 80% at 0.1 mM, and 98% at 0.2 mM

Pelobacter acetylenicus

KCN

20% inhibition at 1 mM, 40% inhibition at 5-10 mM

Pelobacter acetylenicus

molybdate

-

Pelobacter acetylenicus

additional information

no inhibition by citrate, ascorbate, tiron, ferrocene, and EDTA, no inhibition by ethylene at 8 mM

Pelobacter acetylenicus

nitric oxide

complete inhibition at 3 mM

Pelobacter acetylenicus

additional information

-

additional information

the enzyme activity depends on the redox status, overview

Pelobacter acetylenicus

0.014

-

Acetylene

pH 7.0, 30°C

Pelobacter acetylenicus

Iron-sulfur cluster

dependent on, the enzyme is a tungsten/iron-sulfur protein, 4.8 mol of iron per mol of enzyme and 3.9 mol od acid-labile sulfur per mol of enzyme

Pelobacter acetylenicus

additional information

no molybdenum detectable

Pelobacter acetylenicus

Tungsten

dependent on, the enzyme is a tungsten/iron-sulfur protein, 0.4 mol of tungsten per mol of enzyme

Pelobacter acetylenicus

60000

-

gel filtration

Pelobacter acetylenicus

73000

-

1 * 73000, SDS-PAGE

Pelobacter acetylenicus

acetylene + H2O

Pelobacter acetylenicus

-

acetaldehyde

-

?

acetylene + H2O

Pelobacter acetylenicus WoAcy1 / DSM 3246

-

acetaldehyde

-

?

under air at room temperature, native enzyme 240-fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and a second anion exchange chromatography step to homogeneity

Pelobacter acetylenicus

culture condition:acetylene-grown cell

-

Pelobacter acetylenicus

-

culture condition:molybdate-grown cell

-

Pelobacter acetylenicus

-

culture condition:tungstate-grown cell

-

Pelobacter acetylenicus

-

69.2

-

purified enzyme

Pelobacter acetylenicus

enzyme activity is stable even after prolonged storage of the cell extract or of the purified protein under air

Pelobacter acetylenicus

acetylene + H2O

-

669072

Pelobacter acetylenicus

acetaldehyde

-

?

acetylene + H2O

-

669072

Pelobacter acetylenicus WoAcy1 / DSM 3246

acetaldehyde

-

?

additional information

no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity

669072

Pelobacter acetylenicus

?

-

?

additional information

no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity

669072

Pelobacter acetylenicus WoAcy1 / DSM 3246

?

-

?

monomer

1 * 73000, SDS-PAGE

Pelobacter acetylenicus

50

-

Pelobacter acetylenicus

22

80

-

Pelobacter acetylenicus

6

6.5

-

Pelobacter acetylenicus

4

9

-

Pelobacter acetylenicus

chromatofocusing

-

4.3

748243

Kroneck

Acetylene hydratase a non-re ...

Rhodococcus opacus, Rhodococcus ruber, Rhodobacter capsulatus, Norcadia rhodochorous, Rhodococcus sp. A1, Gordona alkanivorans, Pelobacter acetylenicus

J. Biol. Inorg. Chem.

21

29-38

2016

-

1

5

-

2

-

1

19

-

7

-

7

-

7

-

2

-

14

1

-

14

-

14

1

5

-

2

-

1

19

-

7

-

2

-

14

1

-

9

-

748431

Boll

Structure and function of the ...

Pelobacter acetylenicus

J. Mol. Microbiol. Biotechnol.

26

119-137

2016

-

1

5

-

2

-

1

4

-

1

-

2

-

1

-

2

-

3

-

3

1

5

-

2

-

1

4

-

1

-

2

-

2

-

730116

Liao

Convergence in the QM-only and ...

Escherichia coli

J. Comput. Chem.

34

2389-2397

2013

-

1

-

4

-

1

-

1

-

1

-

2

-

3

-

1

-

4

-

1

-

1

-

2

-

1

-

713603

Liao

-

Theoretical study of the chemo ...

Pelobacter acetylenicus

ACS Catal.

1

937-944

2011

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

2

-

1

-

715374

Tenbrink

Exploring the active site of t ...

Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246

J. Bacteriol.

193

1229-1236

2011

-

1

4

-

1

2

-

2

-

7

-

1

-

2

-

4

1

-

1

-

1

-

1

-

1

4

-

1

2

-

2

-

2

-

4

1

-

1

-

703363

Vincent

A DFT study of the possible ro ...

Pelobacter acetylenicus

Dalton Trans.

39

3816-3822

2010

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

716769

Liao

Mechanism of tungsten-dependen ...

Pelobacter acetylenicus

Proc. Natl. Acad. Sci. USA

107

22523-22527

2010

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

706054

Antony

-

Theoretical studies of models ...

Pelobacter acetylenicus

Organometallics

28

4938-4944

2009

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

670710

Seiffert

Structure of the non-redox-act ...

Pelobacter acetylenicus

Proc. Natl. Acad. Sci. USA

104

3073-3077

2007

-

1

-

2

-

1

-

1

-

1

-

1

-

2

1

-

1

-

2

-

1

-

1

-

2

1

-

667090

Einsle

Crystallization and preliminar ...

Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246

Acta Crystallogr. Sect. F

F61

299-301

2005

2

-

1

-

1

-

2

1

2

-

7

1

-

1

-

1

-

4

1

-

1

-

2

-

1

-

1

-

2

1

2

-

1

-

1

-

1

-

4

1

-

1

-

668453

Meckenstock

Acetylene hydratase of Pelobac ...

Pelobacter acetylenicus

Eur. J. Biochem.

264

176-182

1999

1

-

1

-

3

2

1

-

5

1

-

1

-

1

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

3

2

1

-

1

-

1

-

1

2

-

1

-

1

-

668651

Rosner

Acetylene degradation by new i ...

Rhodococcus opacus, Rhodococcus ruber, Gordonia sp., Rhodococcus ruber aerobic, Gordonia sp. aerobic

FEMS Microbiol. Lett.

148

175-180

1997

2

-

6

-

5

-

14

-

3

-

5

-

2

-

6

-

5

-

3

-

5

-

669039

Yadav

-

A functional mimic of the new ...

Pelobacter acetylenicus

J. Am. Chem. Soc.

119

4315-4316

1997

-

3

1

-

1

-

1

-

2

1

-

1

-

1

-

3

1

-

2

1

-

669072

Rosner

Purification and characterizat ...

Pelobacter acetylenicus, Pelobacter acetylenicus WoAcy1 / DSM 3246

J. Bacteriol.

177

5767-5772

1995

3

-

6

2

-

3

2

-

10

-

1

-

3

1

4

1

-

1

-

1

-

1

-

3

-

6

-

2

-

3

2

-

1

-

3

1

4

1

-

1

-

1

-

Purification and characterization of acetylene hydratase of Pelobacter acetylenicus, a tungsten iron-sulfur protein

Data extracted from this reference: