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Literature summary for 4.2.1.11 extracted from

  • Haferkamp, P.
    Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus (2011), PH. D. Thesis Universität Duisburg-Essen, 2011, 0000.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
0.18
-
2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
0.392
-
phosphoenolpyruvate 80°C, pH not specified in the publication Saccharolobus solfataricus
0.538
-
phosphoenolpyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46700
-
x * 48000-52000, SDS-PAGE, x * 46700, calculated Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97ZJ3
-
-
Saccharolobus solfataricus DSM 1617 Q97ZJ3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Saccharolobus solfataricus phosphoenolpyruvate + H2O
-
r
2-phospho-D-glycerate
-
Saccharolobus solfataricus DSM 1617 phosphoenolpyruvate + H2O
-
r
phosphoenolpyruvate + H2O
-
Saccharolobus solfataricus 2-phospho-D-glycerate
-
r
phosphoenolpyruvate + H2O
-
Saccharolobus solfataricus DSM 1617 2-phospho-D-glycerate
-
r

Subunits

Subunits Comment Organism
? x * 48000-52000, SDS-PAGE, x * 46700, calculated Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
ENO
-
Saccharolobus solfataricus
SSO0913
-
Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
58.44
-
phosphoenolpyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
91.88
-
phosphoenolpyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
110.8
-
phosphoenolpyruvate 80°C, pH not specified in the publication Saccharolobus solfataricus
831.5
-
2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
909.5
-
2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
941.9
-
2-phospho-D-glycerate 88°C, pH not specified in the publication Saccharolobus solfataricus