Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | inhibitory at higher concentrations | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03205 | - |
2-phospho-D-glycerate | isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.04482 | - |
2-phospho-D-glycerate | isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.05856 | - |
2-phospho-D-glycerate | isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enolase is activated by low concentrations of Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46672 | - |
x * 46672, isoform Eno1p, calculated from amino acid sequence | Saccharomyces cerevisiae |
46673 | - |
x * 46673, isoform Eno1p, electrospray ionization mass spectrometry | Saccharomyces cerevisiae |
47181 | - |
x * 47181, isoform Err3p, calculated from amino acid sequence | Saccharomyces cerevisiae |
47184 | - |
x * 47184, isoform Err3p, electrospray ionization mass spectrometry | Saccharomyces cerevisiae |
47196 | - |
x * 47196, isoform Err2p, calculated from amino acid sequence | Saccharomyces cerevisiae |
47198 | - |
x * 47198, isoform Err2p, electrospray ionization mass spectrometry | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Saccharomyces cerevisiae | - |
phosphoenolpyruvate + H2O | - |
? | |
2-phospho-D-glycerate | Saccharomyces cerevisiae BY4743 | - |
phosphoenolpyruvate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4743 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Saccharomyces cerevisiae | phosphoenolpyruvate + H2O | - |
? | |
2-phospho-D-glycerate | - |
Saccharomyces cerevisiae BY4743 | phosphoenolpyruvate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46672, isoform Eno1p, calculated from amino acid sequence | Saccharomyces cerevisiae |
? | x * 46673, isoform Eno1p, electrospray ionization mass spectrometry | Saccharomyces cerevisiae |
? | x * 47181, isoform Err3p, calculated from amino acid sequence | Saccharomyces cerevisiae |
? | x * 47184, isoform Err3p, electrospray ionization mass spectrometry | Saccharomyces cerevisiae |
? | x * 47196, isoform Err2p, calculated from amino acid sequence | Saccharomyces cerevisiae |
? | x * 47198, isoform Err2p, electrospray ionization mass spectrometry | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Eno1p | - |
Saccharomyces cerevisiae |
enolase | - |
Saccharomyces cerevisiae |
Err2p | - |
Saccharomyces cerevisiae |
Err3p | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55.5 | - |
the melting temperature of isoform Eno1p, Err2, and Err3p are 55.5°C, 60.5°C, and 60°C, respectively | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.4 | - |
2-phospho-D-glycerate | isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae | |
19.7 | - |
2-phospho-D-glycerate | isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae | |
71.4 | - |
2-phospho-D-glycerate | isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.327 | - |
Mg2+ | isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.695 | - |
Mg2+ | isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae | |
2.1 | - |
Mg2+ | isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication | Saccharomyces cerevisiae |