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Literature summary for 4.2.1.11 extracted from

  • Floden, A.M.; Watt, J.A.; Brissette, C.A.
    Borrelia burgdorferi enolase is a surface-exposed plasminogen binding protein (2011), PLoS ONE, 6, e27502.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3)pLysS cells Borreliella burgdorferi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Borreliella burgdorferi 5737
-
outer surface
-
 Borreliella burgdorferi
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Borreliella burgdorferi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Borreliella burgdorferi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Borreliella burgdorferi
-
 phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate Borreliella burgdorferi B31-MI-16
-
 phosphoenolpyruvate + H2O
-
 ?
additional information Borreliella burgdorferi plasminogen bound to recombinant enolase can be converted to active plasmin ?
-
 ?
additional information Borreliella burgdorferi B31-MI-16 plasminogen bound to recombinant enolase can be converted to active plasmin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Borreliella burgdorferi
-
-
-
Borreliella burgdorferi B31-MI-16
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MagneHis nickel-conjugated magnetic bead column chromatography, and gel filtration Borreliella burgdorferi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Borreliella burgdorferi phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate
-
 Borreliella burgdorferi B31-MI-16 phosphoenolpyruvate + H2O
-
 ?
additional information plasminogen bound to recombinant enolase can be converted to active plasmin Borreliella burgdorferi ?
-
 ?
additional information plasminogen bound to recombinant enolase can be converted to active plasmin Borreliella burgdorferi B31-MI-16 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Borreliella burgdorferi

Synonyms

Synonyms Comment Organism
enolase
-
 Borreliella burgdorferi

pI Value

Organism Comment pI Value Maximum pI Value
Borreliella burgdorferi calculated from amino acid sequence
-
 5.4

General Information

General Information Comment Organism
physiological function enolase is a moonlighting cytoplasmic protein which also associates with the bacterial outer surface and facilitates binding to host plasminogen Borreliella burgdorferi