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Literature summary for 4.2.1.11 extracted from

  • Jiang, W.; Han, X.; Wang, Q.; Li, X.; Yi, L.; Liu, Y.; Ding, C.
    Vibrio parahaemolyticus enolase is an adhesion-related factor that binds plasminogen and functions as a protective antigen (2014), Appl. Microbiol. Biotechnol., 98, 4937-4948.
    View publication on PubMed
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Vibrio parahaemolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 2-phospho-D-glycerate in 100 mM HEPES buffer, pH 8.5, 7.7 mM KCl, 10 mM MgSO4, at 25°C Vibrio parahaemolyticus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Vibrio parahaemolyticus 5737
-
extracellular
-
 Vibrio parahaemolyticus
-
-
outer membrane
-
 Vibrio parahaemolyticus 19867
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, calculated from amino acid sequence Vibrio parahaemolyticus
52000
-
 x * 52000, His6-tagged enzyme, SDS-PAGE Vibrio parahaemolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Vibrio parahaemolyticus
-
 phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate Vibrio parahaemolyticus ATCC 33847
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio parahaemolyticus W6ABG9
-
-
Vibrio parahaemolyticus ATCC 33847 W6ABG9
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind column chromatography Vibrio parahaemolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Vibrio parahaemolyticus phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate
-
 Vibrio parahaemolyticus ATCC 33847 phosphoenolpyruvate + H2O
-
 ?
additional information the enzyme binds to plasminogen Vibrio parahaemolyticus ?
-
 ?
additional information the enzyme binds to plasminogen Vibrio parahaemolyticus ATCC 33847 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, calculated from amino acid sequence Vibrio parahaemolyticus
? x * 52000, His6-tagged enzyme, SDS-PAGE Vibrio parahaemolyticus

Synonyms

Synonyms Comment Organism
enolase
-
 Vibrio parahaemolyticus