Cloned (Comment) | Organism |
---|---|
expression of His-tagged enolase in Escherichia coli strain BL21 | Brucella abortus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cr2+ | leads to complete inhibition at 10 mM | Brucella abortus | |
Cu2+ | leads to complete inhibition at 10 mM | Brucella abortus | |
Hg2+ | leads to complete inhibition at 10 mM | Brucella abortus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
2-phospho-D-glycerate | pH 8.5, 25°C, recombinant enzyme | Brucella abortus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | can partially substitute for Mg2+ or Zn2+ at 10 mM showing 68.8% of the maximal activity with 10 mM Mg2+ | Brucella abortus | |
Mg2+ | activates, optimally at 10 mM | Brucella abortus | |
Mn2+ | can partially substitute for Mg2+ or Zn2+ at 10 mM showing 90.4% of the maximal activity with 10 mM Mg2+ | Brucella abortus | |
additional information | 57% of maximal activity without metal ion compared to Mg2+ at 10 mM | Brucella abortus | |
Ni2+ | can partially substitute for Mg2+ or Zn2+ at 10 mM showing 90.5% of the maximal activity with 10 mM Mg2+ | Brucella abortus | |
Zn2+ | activates, optimally at 10 mM, best activating metal ion | Brucella abortus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, recombinant His-tagged enolase, SDS-PAGE | Brucella abortus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Brucella abortus | - |
phosphoenolpyruvate + H2O | - |
? | |
2-phospho-D-glycerate | Brucella abortus A19 | - |
phosphoenolpyruvate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella abortus | Q2YPV0 | strain 2308, 100% sequence identity with strain A19 | - |
Brucella abortus A19 | Q2YPV0 | strain 2308, 100% sequence identity with strain A19 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enolase from Escherichia coli strain BL21 by nickel affinity chromatography | Brucella abortus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3909 | - |
purifed recombinant enzyme, pH 8.5, 37°C, no metal ion added | Brucella abortus |
9091 | - |
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Mg2+ | Brucella abortus |
10050 | - |
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Zn2+ | Brucella abortus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Brucella abortus | phosphoenolpyruvate + H2O | - |
? | |
2-phospho-D-glycerate | - |
Brucella abortus A19 | phosphoenolpyruvate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, recombinant His-tagged enolase, SDS-PAGE | Brucella abortus |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Brucella abortus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Brucella abortus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 62 | maximally stable at 37°C, the purified recombinant enolase is thermally stable at 25°C, 37°C, 50°C, and 62°C, but is completely inhibited by a preincubation of the enzyme at 75°C after 1 h | Brucella abortus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant enzyme | Brucella abortus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, recombinant enzyme | Brucella abortus |
General Information | Comment | Organism |
---|---|---|
physiological function | the recombinant enolase exhibits fibronectin-binding ability in immunoblotting assay, suggesting that enolase may play a role in Brucella abortus colonization, persistence, and invasion of host tissue | Brucella abortus |