Any feedback?
Literature summary for 4.2.1.11 extracted from
- Aphidius ervi teratocytes release an extracellular enolase (2009), Insect Biochem. Mol. Biol., 39, 801-813.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosettagami (DE3) cells | Aphidius ervi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.086 | - |
2-phospho-D-glycerate | in 81 mM triethanolamine/HCl, pH 7.4, at 25°C | Aphidius ervi |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aphidius ervi | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the highest activation rate of the enzymatic activity is reached at 5 mM Mg2+ and remains unchanged up to 25 mM | Aphidius ervi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer | Aphidius ervi |
| 98000 | - |
gel filtration | Aphidius ervi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aphidius ervi | C9YSH7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA-agarose column chromatography, gel filtration | Aphidius ervi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Aphidius ervi | - |
| larva | - |
Aphidius ervi | - |
| teratocyte | present in the teratocytes and released in the host haemocoel | Aphidius ervi | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Aphidius ervi | phosphoenolpyruvate + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer | Aphidius ervi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ENO | - |
Aphidius ervi |
| enolase | - |
Aphidius ervi |
| p45 | - |
Aphidius ervi |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
