Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Homo sapiens | |
Mg2+ | dependent on | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Homo sapiens |
47000 | - |
2 * 47000, SDS-PAGE | Klebsiella pneumoniae |
94000 | - |
native enzyme, gel filtration | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE Sephadex A-50 gel filtration, Sephadex G-100 gel filtration | Klebsiella pneumoniae |
ammonium sulfate precipitation, DEAE-Sephadex A-50 gel filtration, CM-Sephadex gel filtration, and QAE-Sephadex gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.41 | - |
crude extract, at pH 7.8 | Klebsiella pneumoniae |
0.9 | - |
crude extract, at pH 6.8 | Homo sapiens |
31.2 | - |
after 76.1fold purification, at pH 7.8 | Klebsiella pneumoniae |
75 | - |
after 83fold purification, at pH 6.8 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Homo sapiens | phosphoenolpyruvate + H2O | - |
r | |
2-phospho-D-glycerate | - |
Klebsiella pneumoniae | phosphoenolpyruvate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Homo sapiens |
homodimer | 2 * 47000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
2-phosphopyruvate hydrolyase | - |
Homo sapiens |
2-phosphopyruvate hydrolyase | - |
Klebsiella pneumoniae |
enolase | - |
Homo sapiens |
enolase | - |
Klebsiella pneumoniae |