Literature summary for 4.2.1.11 extracted from

Kolberg, J.; Aase, A.; Bergmann, S.; Herstad, T.K.; Rodal, G.; Frank, R.; Rohde, M.; Hammerschmidt, S.
Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface (2006), Microbiology, 152, 1307-1317.

Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli host strain M15-pREP4, His-tagged protein	Streptococcus pneumoniae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	low amounts of surface-exposed enolase, determined on viable pneumococci, monoclonal and polyconal antibodies used	Streptococcus pneumoniae	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Streptococcus pneumoniae	-	phosphoenolpyruvate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	Q97QS2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, for epitope mapping and identification of plasminogen-binding motifs	Streptococcus pneumoniae

Source Tissue

Source Tissue	Comment	Organism	Textmining
bacteroid	-	Streptococcus pneumoniae	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	purified recombinant enolase has plasminogen binding activity, analyzed by flow cytometry	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Streptococcus pneumoniae	phosphoenolpyruvate + H2O	-	r
2-phospho-D-glycerate	plasminogen-binding activity besides metabolic function	Streptococcus pneumoniae	phosphoenolpyruvate + H2O	-	r

Subunits

Subunits	Comment	Organism
More	epitope for plasminogen-binding localized in a surface-exposed loop in each of the monomers of the octameric enolase	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
enolase	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)