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Literature summary for 4.2.1.11 extracted from

  • Rodriguez, E.; Romaris, F.; Lorenzo, S.; Moreno, J.; Bonay, P.; Ubeira, F.M.; Garate, T.
    A recombinant enolase from Anisakis simplex is differentially recognized in natural human and mouse experimental infections (2006), Med. Microbiol. Immunol., 195, 1-10.
    View publication on PubMed
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
cDNA inserted in PGEX-4T-3 expressed in Escherichia coli, glutathione S-transferase fusion protein, protein with 436 amino acid residues, 70-80% sequence similarity to enolases from other organisms, including helminth parasites Anisakis simplex

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Anisakis simplex
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Anisakis simplex Q8MU59
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose-4B chromatography Anisakis simplex

Source Tissue

Source Tissue Comment Organism Textmining
larva L3 state Anisakis simplex
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 functional activity of recombinant protein shown, immunogenicity determined by ELISA and Western Blot using different sera, natural infection of humans by Anisakis simplex larvae lacks sufficient antigenic stimuli Anisakis simplex

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Anisakis simplex phosphoenolpyruvate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
enolase
-
 Anisakis simplex