Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of a his-tagged recombinant enzyme | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
hanging drop method | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
2-phospho-D-glycerate | pH 7.6, 25ºC | Trypanosoma brucei | |
0.244 | - |
phosphoenolpyruvate | pH 7.6, 25ºC | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma brucei | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for activity, high concentrations are inhibitory | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46461 | - |
x * 46461, calculated from amino-acid sequence | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q9NDH8 | - |
- |
Purification (Comment) | Organism |
---|---|
using affinity chromatography to isolate a his-tagged recombinant enzyme followed by cleavage of the tag | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
pH 7.6, 25ºC | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Trypanosoma brucei | phosphoenolpyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 46461, calculated from amino-acid sequence | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
2-phospho-D-glycerate hydrolase | - |
Trypanosoma brucei |
enolase | - |
Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
25ºC | Trypanosoma brucei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8 | activity decreases steadily, 60% of the activity remains at pH 7.0 and pH 8.4 | Trypanosoma brucei |