Cloned (Comment) | Organism |
---|---|
expression of recombinant wild-type and mutant enzymes in Escherichia coli | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
E414L | this mutant has the same activity than the wild-type enzyme | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaClO4 | E414L mutant is more sensitive to inactivation than the wild-type enzyme | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
phosphoenolpyruvate | E414L mutant enzyme, pH 7.1, 25ºC | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46940 | - |
mass spectrometry | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P25704 | rabbit | - |
Purification (Comment) | Organism |
---|---|
purification of the wild-type and a mutant enzyme cloned in Escherichia coli using ammonium sulfate precipitation and Sephadex chromatography | Oryctolagus cuniculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67 | - |
recombinant wild-type enzyme, pH 7.1, 25ºC | Oryctolagus cuniculus |
77 | - |
recombinant E414L mutant, pH 7.1, 25ºC | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Oryctolagus cuniculus | phosphoenolpyruvate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
both the wild-type and the E414L mutant are stable as suspensions in ammonium sulfate | Oryctolagus cuniculus |