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Literature summary for 4.2.1.11 extracted from

  • Kornblatt, M.J.; Zheng, S.X.; Lamande, N.; Lazar, M.
    Cloning, expression and mutagenesis of a subunit contact of rabbit muscle-specific (betabeta) enolase (2002), Biochim. Biophys. Acta, 1597, 311-319.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant wild-type and mutant enzymes in Escherichia coli Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
E414L this mutant has the same activity than the wild-type enzyme Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
NaClO4 E414L mutant is more sensitive to inactivation than the wild-type enzyme Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
43
-
phosphoenolpyruvate E414L mutant enzyme, pH 7.1, 25ºC Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46940
-
mass spectrometry Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus P25704 rabbit
-

Purification (Commentary)

Purification (Comment) Organism
purification of the wild-type and a mutant enzyme cloned in Escherichia coli using ammonium sulfate precipitation and Sephadex chromatography Oryctolagus cuniculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67
-
recombinant wild-type enzyme, pH 7.1, 25ºC Oryctolagus cuniculus
77
-
recombinant E414L mutant, pH 7.1, 25ºC Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Oryctolagus cuniculus phosphoenolpyruvate
-
r

Synonyms

Synonyms Comment Organism
enolase
-
Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
both the wild-type and the E414L mutant are stable as suspensions in ammonium sulfate Oryctolagus cuniculus