Any feedback?
Literature summary for 4.2.1.11 extracted from
- Enolase from spores and cells of Bacillus megaterium: two-step purification of the enzyme and some of its properties (1978), J. Bacteriol., 134, 353-355.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Priestia megaterium |  |
| F- | - |
Priestia megaterium | |
| additional information | no inhibition by NEM and iodoacetate | Priestia megaterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.71 | - |
2-phospho-D-glycerate | - |
Priestia megaterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Priestia megaterium | |
| Mg2+ | Km: 0.47 mM | Priestia megaterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
8 * 42000, SDS-PAGE | Priestia megaterium |
| 335000 | - |
gel filtration | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Priestia megaterium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spore | germinated | Priestia megaterium | - |
| vegetative | - |
Priestia megaterium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Priestia megaterium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| in the frozen state, stable for 6 months | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Priestia megaterium | phosphoenolpyruvate | - |
? | |
| 2-phospho-D-glycerate | - |
Priestia megaterium | phosphoenolpyruvate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 42000, SDS-PAGE | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Priestia megaterium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
