BRENDA - Reference to 4.2.1.108; Id = 746650

gene ectC, recombinant expression of a codon-optimized version of C-terminall Strep II-tagged enzyme in Escherichia coli strain BL21 under control of the tet promoter, which in turn is controlled by the TetR repressor, the genetic expression system can be induced by adding anhydrotetracycline

Sphingopyxis alaskensis

purified recombinant Strep II-tagged enzyme, sitting drop vapour diffusion method, mixing of 100 nl of 11 mg/ml enzyme solution with 100 nl of reservoir solution containing 0.05 M, calcium acetate, 0.1 M sodium acetate, pH 4.5, and 40% v/v 1,2-propanediol, and equilibration against 0.05 ml of reservoir solution, 12°C, ten weeks, X-ray diffraction structure determination and analysis at 1.0 A resolution, molecular replacement using the crystal structure of Zn2+-containing cupin RemF from Streptomyces resistomycificus (PDB ID 3ht1) as template

Sphingopyxis alaskensis

32700

-

recombinant Strep II-tagged enzyme, gel filtration

Sphingopyxis alaskensis

(2S)-4-acetamido-2-aminobutanoate

Sphingopyxis alaskensis

-

L-ectoine + H2O

-

?

(2S)-4-acetamido-2-aminobutanoate

Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256

-

L-ectoine + H2O

-

?

Sphingopyxis alaskensis

Q1GNW6

i.e. Sphingomonas alaskensis

-

Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256

Q1GNW6

i.e. Sphingomonas alaskensis

-

recombinant C-terminall Strep II-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration to homogeneity

Sphingopyxis alaskensis

(2S)-4-acetamido-2-aminobutanoate

-

746650

Sphingopyxis alaskensis

L-ectoine + H2O

-

?

(2S)-4-acetamido-2-aminobutanoate

-

746650

Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256

L-ectoine + H2O

-

?

dimer

-

Sphingopyxis alaskensis

EctC

-

Sphingopyxis alaskensis

L-ectoine synthase

UniProt

Sphingopyxis alaskensis

SaEctC

-

Sphingopyxis alaskensis

gene ectC, recombinant expression of a codon-optimized version of C-terminall Strep II-tagged enzyme in Escherichia coli strain BL21 under control of the tet promoter, which in turn is controlled by the TetR repressor, the genetic expression system can be induced by adding anhydrotetracycline

Sphingopyxis alaskensis

purified recombinant Strep II-tagged enzyme, sitting drop vapour diffusion method, mixing of 100 nl of 11 mg/ml enzyme solution with 100 nl of reservoir solution containing 0.05 M, calcium acetate, 0.1 M sodium acetate, pH 4.5, and 40% v/v 1,2-propanediol, and equilibration against 0.05 ml of reservoir solution, 12°C, ten weeks, X-ray diffraction structure determination and analysis at 1.0 A resolution, molecular replacement using the crystal structure of Zn2+-containing cupin RemF from Streptomyces resistomycificus (PDB ID 3ht1) as template

Sphingopyxis alaskensis

32700

-

recombinant Strep II-tagged enzyme, gel filtration

Sphingopyxis alaskensis

(2S)-4-acetamido-2-aminobutanoate

Sphingopyxis alaskensis

-

L-ectoine + H2O

-

?

(2S)-4-acetamido-2-aminobutanoate

Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256

-

L-ectoine + H2O

-

?

recombinant C-terminall Strep II-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration to homogeneity

Sphingopyxis alaskensis

(2S)-4-acetamido-2-aminobutanoate

-

746650

Sphingopyxis alaskensis

L-ectoine + H2O

-

?

(2S)-4-acetamido-2-aminobutanoate

-

746650

Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256

L-ectoine + H2O

-

?

dimer

-

Sphingopyxis alaskensis

evolution

ectoine biosynthetic genes (ectABC) are widely distributed in bacteria, ectoine synthase is a member of the cupin superfamily

Sphingopyxis alaskensis

physiological function

microorganisms that carry the ectoine biosynthetic genes (ectABC) make copious amounts of ectoine as a cell protectant in response to high-osmolarity challenges. Ectoine synthase (EctC) is the key enzyme for the production of this compatible solute and mediates the last step of ectoine biosynthesis. It catalyzes the ring closure of the cyclic ectoine molecule

Sphingopyxis alaskensis

evolution

ectoine biosynthetic genes (ectABC) are widely distributed in bacteria, ectoine synthase is a member of the cupin superfamily

Sphingopyxis alaskensis

physiological function

microorganisms that carry the ectoine biosynthetic genes (ectABC) make copious amounts of ectoine as a cell protectant in response to high-osmolarity challenges. Ectoine synthase (EctC) is the key enzyme for the production of this compatible solute and mediates the last step of ectoine biosynthesis. It catalyzes the ring closure of the cyclic ectoine molecule

Sphingopyxis alaskensis

749081

Widderich

Biochemistry and crystal stru ...

Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256

PLoS ONE

11

e0151285

2016

-

1

26

-

3

-

2

-

2

-

7

-

1

-

8

2

5

1

-

2

1

-

1

-

1

26

-

3

-

2

-

2

-

1

-

8

2

1

-

2

1

-

2

-

2

746650

Kobus

Overproduction, crystallizati ...

Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593 / LMG 18877 / RB2256

Acta Crystallogr. Sect. F

71

1027-1032

2015

-

1

-

1

2

-

7

-

1

-

2

1

4

-

1

-

1

-

1

2

-

1

-

2

1

-

2

-

713911

Witt

Unexpected property of ectoine ...

Halomonas elongata, Halomonas elongata DSM 2581

Appl. Microbiol. Biotechnol.

91

113-122

2011

-

1

-

1

-

1

-

1

2

-

5

-

1

-

2

-

11

1

-

1

-

1

-

1

-

1

-

1

2

-

1

-

2

-

11

1

-

1

-

3

-

729687

Reshetnikov

Diversity and phylogeny of the ...

Methylarcula marina, Methylarcula marina ML1, Methylobacter marinus, Methylobacter marinus 7C, Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum ML1, Methylomicrobium kenyense, Methylomicrobium kenyense AMO1, Methylophaga alcalica, Methylophaga alcalica M8, Methylophaga thalassica, Methylophaga thalassica ATCC 33146

Extremophiles

15

653-663

2011

-

6

-

19

-

6

-

6

-

690643

Rajan

Characterization and phylogene ...

Bacillus halodurans, Bacillus halodurans TCJAR021

Arch. Microbiol.

190

481-487

2008

-

1

-

1

4

-

3

-

4

-

1

-

1

-

1

-

1

4

-

4

-

1

-

691437

Rajan

Cloning and heterologous expre ...

Bacillus halodurans, Bacillus halodurans TCJAR021

Biotechnol. Lett.

30

1403-1407

2008

-

1

-

1

4

-

3

-

4

-

1

-

1

-

1

-

1

4

-

4

-

1

-

660836

Reshetnikov

Characterization of the ectoin ...

Methylomicrobium alcaliphilum

Arch. Microbiol.

184

286-297

2006

1

-

1

-

1

-

1

-

3

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

660740

Kuhlmann

Osmotically regulated synthesi ...

Bacillus alcalophilus, Chromohalobacter salexigens, no activity in Aneurinibacillus aneurinilyticus, no activity in Aneurinibacillus aneurinilyticus DSM 5562, no activity in Bacillus cereus, no activity in Bacillus cereus DSM 31, no activity in Bacillus circulans, no activity in Bacillus licheniformis, no activity in Bacillus megaterium, no activity in Bacillus subtilis, no activity in Bacillus subtilis JH642, no activity in Bacillus thuringiensis, no activity in Paenibacillus polymyxa, Sporosarcina pasteurii, Sporosarcina psychrophila, Virgibacillus pantothenticus, Virgibacillus salexigens

Appl. Environ. Microbiol.

68

772-783

2002

6

-

2

-

1

-

12

-

27

-

18

-

6

-

2

-

1

-

12

-

18

-

663114

Nakayama

Ectoine, the compatible solute ...

Halomonas elongata, Halomonas elongata OUT30018, no activity in Nicotiana tabacum

Plant Physiol.

122

1239-1247

2000

-

1

-

1

-

2

-

9

-

4

-

1

-

1

-

2

-

4

-

640091

Ono

Characterization of biosynthet ...

Halomonas elongata, Halomonas elongata OUT30018

J. Bacteriol.

181

91-99

1999

-

1

-

2

-

2

3

-

5

-

1

-

1

-

6

1

-

3

1

-

1

-

1

-

1

-

2

-

2

3

-

1

-

1

-

6

1

3

1

-

1

-

1

-

662773

Louis

Characterization of genes for ...

Marinococcus halophilus

Microbiology

143

1141-1149

1997

1

-

2

-

3

-

3

-

1

-

2

-

3

-

640090

Peters

-

The biosynthesis of ectoine ...

Halomonas elongata, Halorhodospira halochloris

FEMS Microbiol. Lett.

71

157-162

1990

-

2

-

2

-

2

-

4

-

2

-

2

-

2

-

2

-

4

-

2

-

2

-

Overproduction, crystallization and X-ray diffraction data analysis of ectoine synthase from the cold-adapted marine bacterium Sphingopyxis alaskensis

Data extracted from this reference: