Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.106 extracted from

  • Bhowmik, S.; Chiu, H.P.; Jones, D.H.; Chiu, H.J.; Miller, M.D.; Xu, Q.; Farr, C.L.; Ridlon, J.M.; Wells, J.E.; Elsliger, M.A.; Wilson, I.A.; Hylemon, P.B.; Lesley, S.A.
    Structure and functional characterization of a bile acid 7alpha dehydratase BaiE in secondary bile acid synthesis (2016), Proteins, 84, 316-331.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.106

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Peptacetobacter hiranonis
-
 [Clostridium] hylemonae
-
 [Clostridium] scindens

Protein Variants

Protein Variants Comment Organism
D106N inactive mutant [Clostridium] scindens
D35N inactive mutant [Clostridium] scindens
H83N inactive mutant [Clostridium] scindens
R146Q inactive mutant [Clostridium] scindens
Y126F strongly reduced activity compared to wild type [Clostridium] scindens
Y30F inactive mutant [Clostridium] scindens
Y54A inactive mutant [Clostridium] scindens
Y54F reduced activity compared to wild type [Clostridium] scindens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] scindens
4
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl pH 7.3 [Clostridium] scindens
9.9
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl pH 7.3 [Clostridium] scindens
11
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] scindens
11.4
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] hylemonae
21
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] hylemonae
25
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 Peptacetobacter hiranonis
208
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 Peptacetobacter hiranonis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA Peptacetobacter hiranonis
-
 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [Clostridium] hylemonae
-
 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [Clostridium] scindens
-
 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [Clostridium] scindens ATCC 35704
-
 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA Peptacetobacter hiranonis DSM 13275
-
 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [Clostridium] hylemonae DSM 15053
-
 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [Clostridium] scindens VPI 12708
-
 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Peptacetobacter hiranonis Q9RB47
-
-
Peptacetobacter hiranonis DSM 13275 Q9RB47
-
-
[Clostridium] hylemonae B4YSU1
-
-
[Clostridium] hylemonae DSM 15053 B4YSU1
-
-
[Clostridium] scindens B0NI18
-
-
[Clostridium] scindens P19412
-
-
[Clostridium] scindens ATCC 35704 B0NI18
-
-
[Clostridium] scindens VPI 12708 P19412
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7alpha-hydroxy-3-oxochol-4-en-24-oate
-
 Peptacetobacter hiranonis 3-oxochola-4,6-dien-24-oate
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oate
-
 [Clostridium] hylemonae 3-oxochola-4,6-dien-24-oate
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oate
-
 [Clostridium] scindens 3-oxochola-4,6-dien-24-oate
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oate
-
 [Clostridium] scindens ATCC 35704 3-oxochola-4,6-dien-24-oate
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oate
-
 Peptacetobacter hiranonis DSM 13275 3-oxochola-4,6-dien-24-oate
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oate
-
 [Clostridium] hylemonae DSM 15053 3-oxochola-4,6-dien-24-oate
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oate
-
 [Clostridium] scindens VPI 12708 3-oxochola-4,6-dien-24-oate
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
-
 Peptacetobacter hiranonis 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
-
 [Clostridium] hylemonae 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
-
 [Clostridium] scindens 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
-
 [Clostridium] scindens ATCC 35704 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
-
 Peptacetobacter hiranonis DSM 13275 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
-
 [Clostridium] hylemonae DSM 15053 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
-
 [Clostridium] scindens VPI 12708 3-oxochola-4,6-dien-24-oyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
baiE
-
 Peptacetobacter hiranonis
baiE
-
 [Clostridium] hylemonae
baiE
-
 [Clostridium] scindens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 Peptacetobacter hiranonis
12.6
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] hylemonae
15
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] scindens
18
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] scindens
18.6
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] hylemonae
22.2
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 Peptacetobacter hiranonis
24
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl pH 7.3 [Clostridium] scindens
98
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl pH 7.3 [Clostridium] scindens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 Peptacetobacter hiranonis
0.2
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 Peptacetobacter hiranonis
0.9
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] hylemonae
1.1
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] hylemonae
1.6
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] scindens
2.4
-
 7alpha-hydroxy-3-oxochol-4-en-24-oate 25°C in 20 mM Tris-HCl pH 7.3 [Clostridium] scindens
12.5
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl, pH 7.3 [Clostridium] scindens
24.5
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA 25°C in 20 mM Tris-HCl pH 7.3 [Clostridium] scindens