Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.106 extracted from

  • Dawson, J.A.; Mallonee, D.H.; Bjorkhem, I.; Hylemon, P.B.
    Expression and characterization of a C24 bile acid 7alpha-dehydratase from Eubacterium sp. strain VPI 12708 in Escherichia coli (1996), J. Lipid Res., 37, 1258-1267.
    View publication on PubMed
Search for more literature for 4.2.1.106

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Eubacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 7alpha,12alpha-dihydroxy-3-oxochol-4-enoate pH 7.5 Eubacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19500
-
 2 * 19500, deduced from nucleotide sequence Eubacterium sp.
23000
-
 2 * 23000, SDS-PAGE Eubacterium sp.
36000
-
 gel filtration Eubacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate Eubacterium sp.
-
 12alpha-hydroxy-3-oxocholate-4,6-dienoate
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate Eubacterium sp. VPI 12708
-
 12alpha-hydroxy-3-oxocholate-4,6-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Eubacterium sp.
-
-
-
Eubacterium sp. VPI 12708
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE-52, Phenyl-Sepharose, HPLC-DEAE, gel filtration Eubacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
-
 Eubacterium sp. 12alpha-hydroxy-3-oxocholate-4,6-dienoate
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
-
 Eubacterium sp. VPI 12708 12alpha-hydroxy-3-oxocholate-4,6-dienoate
-
 ?
7alpha-hydroxy-3-oxochol-4-enoate 84% of activity with 7alpha,12alpha-dihydroxy-3-oxochol-4-enoate Eubacterium sp. 3-oxocholate-4,6-dienoate
-
 ?
7alpha-hydroxy-3-oxochol-4-enoate 84% of activity with 7alpha,12alpha-dihydroxy-3-oxochol-4-enoate Eubacterium sp. VPI 12708 3-oxocholate-4,6-dienoate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE Eubacterium sp.
dimer 2 * 19500, deduced from nucleotide sequence Eubacterium sp.