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Literature summary for 4.2.1.104 extracted from

  • Kamennaya, N.A.; Post, A.F.
    Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp (2011), Appl. Environ. Microbiol., 77, 291-301.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.104

Cloned(Commentary)

Cloned (Comment) Organism
gene cynS, cynS is presumably transcribed as part of the cynABDS operon, DNA and amino acid sequence determination and analysis, phylogenetic analysis Prochlorococcus sp.
gene cynS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in Escherichia coli strain HMS174 as maltose-binding-protein fusion protein Synechococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Na-azide
-
 Prochlorococcus sp.
Na-azide
-
 Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, recombinant MBP-CynS fusion enzyme, SDS-PAGE Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanate + HCO3- + 2 H+ Synechococcus sp.
-
 NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+ Prochlorococcus sp.
-
 NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+ Prochlorococcus sp. MED4
-
 NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+ Synechococcus sp. WH7803
-
 NH3 + 2 CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Prochlorococcus sp. Q7V2U0 gene cynS
-
Prochlorococcus sp. MED4 Q7V2U0 gene cynS
-
Synechococcus sp. A5GPQ6 gene cynS
-
Synechococcus sp. WH7803 A5GPQ6 gene cynS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CynS-MBP from Escherichia coli strain HMS174 by amylose affinity chromatography Synechococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.43
-
 purified recombinant enzyme, pH 7.4, 55°C Synechococcus sp.
0.8
-
 purified recombinant enzyme, pH 7.4, 4°C Synechococcus sp.
5.56
-
 purified recombinant enzyme, pH 7.4, 26°C Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanate + HCO3- + 2 H+
-
 Synechococcus sp. NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+
-
 Prochlorococcus sp. NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+
-
 Prochlorococcus sp. MED4 NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+
-
 Synechococcus sp. WH7803 NH3 + 2 CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, recombinant MBP-CynS fusion enzyme, SDS-PAGE Synechococcus sp.

Synonyms

Synonyms Comment Organism
cyanate lyase
-
 Synechococcus sp.
CynS
-
 Prochlorococcus sp.
CynS
-
 Synechococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
-
 Synechococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 55 activity range Synechococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 55 CynS is rapidly inactivated at higher temperatures and only residual activity o f 0.09 U/mg occurs at 50°C Synechococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Synechococcus sp.

General Information

General Information Comment Organism
additional information transcriptional regulation of cynS expression, overview Synechococcus sp.
additional information transcriptional regulation of cynS expression, overview Prochlorococcus sp.