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Literature summary for 4.2.1.104 extracted from

  • Luque-Almagro, V.M.; Huertas, M.J.; Saez, L.P.; Luque-Romero, M.M.; Moreno-Vivian, C.; Castillo, F.; Roldan, M.D.; Blasco, R.
    Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an enzyme that is not essential for cyanide assimilation (2008), Appl. Environ. Microbiol., 74, 6280-6288.
    View publication on PubMed
Search for more literature for 4.2.1.104

Activating Compound

Activating Compound Comment Organism Structure
Cyanate positive regulation Ectopseudomonas oleovorans
cyanide positive regulation Ectopseudomonas oleovorans
Urea strong inducer of cyanase activity Ectopseudomonas oleovorans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli DH5 alpha Ectopseudomonas oleovorans

Protein Variants

Protein Variants Comment Organism
additional information mutant lacking the cynS gene, mutant is unable to use cyanate as the sole nitrogen source but shows the same resistance to cyanate as the wild-type strain, cynS-mutant is not affected in its ability to degrade cyanide, which unambiguously indicates that cyanate is not a central metabolite in cyanide assimilation Ectopseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
ammonium negative regulation even in the presence of cyanate. The negative effect exerted by ammonium on cyanate assimilation seems to take place at the level of gene expression, since the addition of ammonium to cells growing in cyanate had no effect on either cyanase activity or cyanate consumption Ectopseudomonas oleovorans
azide
-
 Ectopseudomonas oleovorans
additional information the enzyme is insensitive to 10 mM thiocyanate, a competitive inhibitor of some cyanases Ectopseudomonas oleovorans
nitrate
-
 Ectopseudomonas oleovorans
p-hydroxymercuribenzoate
-
 Ectopseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 CNO-
-
 Ectopseudomonas oleovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Ectopseudomonas oleovorans 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
-
 Ectopseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanate + ? Ectopseudomonas oleovorans
-
 ?
-
 ?
cyanate + ? Ectopseudomonas oleovorans CECT 5344
-
 ?
-
 ?
additional information Ectopseudomonas oleovorans no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation ?
-
 ?
additional information Ectopseudomonas oleovorans CECT 5344 no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ectopseudomonas oleovorans A4GXA0 expression in Escherichia coli DH5 alpha
-
Ectopseudomonas oleovorans CECT 5344 A4GXA0 expression in Escherichia coli DH5 alpha
-

Purification (Commentary)

Purification (Comment) Organism
precipitation, chromatography Ectopseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CNO- + HCO3- + 2 H+
-
 Ectopseudomonas oleovorans NH3 + 2 CO2
-
 ?
CNO- + HCO3- + 2 H+
-
 Ectopseudomonas oleovorans CECT 5344 NH3 + 2 CO2
-
 ?
cyanate + ?
-
 Ectopseudomonas oleovorans ?
-
 ?
cyanate + ?
-
 Ectopseudomonas oleovorans CECT 5344 ?
-
 ?
additional information no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation Ectopseudomonas oleovorans ?
-
 ?
additional information cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source Ectopseudomonas oleovorans ?
-
 ?
additional information no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation Ectopseudomonas oleovorans CECT 5344 ?
-
 ?
additional information cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source Ectopseudomonas oleovorans CECT 5344 ?
-
 ?

Synonyms

Synonyms Comment Organism
cyanase
-
 Ectopseudomonas oleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Ectopseudomonas oleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 the thermostability range represents temperatures at which the enzyme retains more than 25% of its original activity after 30 min. Higher temperatures result in the irreversible inactivation of the enzyme Ectopseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Ectopseudomonas oleovorans