Literature summary for 4.2.1.104 extracted from

Bezsudnova, E.Y.; Sorokin, D.Y.; Tikhonova, T.V.; Popov, V.O.
Thiocyanate hydrolase, the primary enzyme initiating thiocyanate degradation in the novel obligately chemolithoautotrophic halophilic sulfur-oxidizing bacterium Thiohalophilus thiocyanoxidans (2007), Biochim. Biophys. Acta, 1774, 1563-1570.

Search for more literature for 4.2.1.104

Inhibitors

Inhibitors	Comment	Organism	Structure
chloride	kinetics of cyanase activity at pH 7.5 and 37°C	Thiohalophilus thiocyanatoxydans
Cyanate	concentration above 4 mM	Thiohalophilus thiocyanatoxydans
thiocyanate	kinetics of cyanase activity at pH 7.5 and 37°C	Thiohalophilus thiocyanatoxydans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.048	-	Cyanate	cyanate concentrations between 0.2 mM and 1 mM, in presence of 3 mM NaHCO3, at 37°C and pH 7.5	Thiohalophilus thiocyanatoxydans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14000	-	10 * 14000, estimated by gel filtration, SDS-PAGE, similar to apparent masses of cyanase of Escherichia coli	Thiohalophilus thiocyanatoxydans
70000	-	gel filtration	Thiohalophilus thiocyanatoxydans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NCO- + HCO3- + H+	Thiohalophilus thiocyanatoxydans	cyanase activity in Thiohalophilus thiocyanoxidans, role in thiocyanate decomposition suggested	NH3 + CO2	intermediates carbamate and CO2, spontaneous dissociation into NH3 and CO2	?

Organic Solvent Stability

Organic Solvent	Comment	Organism
SDS	stable after 2 h in 5% SDS at 37°C in 0.1 M potassium phosphate buffer	Thiohalophilus thiocyanatoxydans

Organism

Organism	UniProt	Comment	Textmining
Thiohalophilus thiocyanatoxydans	-	halophilic sulfur-oxidizing gamma-proteobacterium	-

Purification (Commentary)

Purification (Comment)	Organism
co-purified with thiocyanate hydrolase of Thiohalophilus thiocyanoxidans, gel filtration and SDS-PAGE	Thiohalophilus thiocyanatoxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity assay in 0.1 M potassium phosphate buffer at pH 7.5 and 37°C with or without 3 mM NaHCO3, cyanate concentration between 0.2 mM and 2 mM, 0.2 to 1 microgram total protein per milliliter, measurement of NH3 formation	Thiohalophilus thiocyanatoxydans
0.01	-	in absence of bicarbonate	Thiohalophilus thiocyanatoxydans
0.22	-	in the presence of 3 mM bicarbonate and 2 mM cyanate	Thiohalophilus thiocyanatoxydans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NCO- + HCO3- + H+	cyanase activity in Thiohalophilus thiocyanoxidans, role in thiocyanate decomposition suggested	Thiohalophilus thiocyanatoxydans	NH3 + CO2	intermediates carbamate and CO2, spontaneous dissociation into NH3 and CO2	?
NCO- + HCO3- + H+	thiocyanate metabolism of Thiohalophilus thiocyanoxidans	Thiohalophilus thiocyanatoxydans	NH3 + CO2	-	?

Subunits

Subunits	Comment	Organism
decamer	10 * 14000, estimated by gel filtration, SDS-PAGE, similar to apparent masses of cyanase of Escherichia coli	Thiohalophilus thiocyanatoxydans

Synonyms

Synonyms	Comment	Organism
cyanase	-	Thiohalophilus thiocyanatoxydans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Thiohalophilus thiocyanatoxydans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Thiohalophilus thiocyanatoxydans

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
additional information	-	kinetics of cyanase activity at pH 7.5 and 37°C, presence of 3 mM NaHCO3, cyanate concentrations between 0.2 and 2 mM	Thiohalophilus thiocyanatoxydans	additional information
6	-	-	Thiohalophilus thiocyanatoxydans	thiocyanate
15	-	-	Thiohalophilus thiocyanatoxydans	chloride

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Release 2023.1 (January 2023)