Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bicarbonate | - |
Escherichia coli | |
Cyanate | extracellular, enzyme induction | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
selenomethionine-labeled purified recombinant enzyme, also crystals of enzyme with complexed inhibitors oxalate and chloride, 2 mM dithiothreitol, sitting drop vapour diffusion method, from 2.1 M ammonium sulfate, 50 mM Na2KPO4, pH 7.3, microseeding with large sitting drops at 18°C with wild-type crystals, 5-7 days, X-ray diffraction structure determinzation and analysis at 1.65 A resolution by multiwavelength anomalous diffraction MAD method | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloride | - |
Escherichia coli | |
oxalate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
10 * 17000, SDS-PAGE | Escherichia coli |
170000 | - |
crystal structure | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene of cyn operon, inducible enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 | the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamae. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide, the decamer shows half-side binding of substrates and substrate analogues, catalytic mechanism, catalytic site involves Arg96, Glu99, and Ser122 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NCO- + HCO3- | - |
Escherichia coli | NH4+ + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 17000, SDS-PAGE | Escherichia coli |
More | the monomers are composed of 2 domains, subunit arrangement, decamer is formed by 5 dimers assembled into a pentamer, model | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
cyanase | - |
Escherichia coli |
EC 4.3.99.1 | formerly | Escherichia coli |