Any feedback?
Literature summary for 4.2.1.103 extracted from
- Novel isonitrile hydratase involved in isonitrile metabolism (2010), J. Biol. Chem., 285, 34793-34802.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| InhB, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH10B | Arthrobacter pascens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 99.8% inhibition at 1 mM | Arthrobacter pascens |  |
| 5,5'-dithiobis-(2-nitrobenzoate) | 34.9% inhibition at 1 mM | Arthrobacter pascens | |
| 8-hydroxyquinoline | 26.1% inhibition at 1 mM | Arthrobacter pascens | |
| Ag+ | complete inhibition at 1 mM | Arthrobacter pascens | |
| Al3+ | 21.4% inhibition at 1 mM | Arthrobacter pascens | |
| Cd2+ | 71.6% inhibition at 1 mM | Arthrobacter pascens | |
| Co2+ | 89.5% inhibition at 1 mM | Arthrobacter pascens | |
| Cu2+ | complete inhibition at 1 mM | Arthrobacter pascens | |
| Fe2+ | 7.6% inhibition at 1 mM | Arthrobacter pascens | |
| Fe3+ | 19.7% inhibition at 1 mM | Arthrobacter pascens | |
| H2O2 | 45.0% inhibition at 1 mM | Arthrobacter pascens | |
| Hg2+ | complete inhibition at 1 mM | Arthrobacter pascens | |
| additional information | the hydrophilic thiol reagent iodoacetate does not influence the enzyme activity. No inhibition by LiCl, NaCl, MgCl, CaCl2, MnCl2, RbCl, CsCl, SrCl2, hydroxylamine, phenylhydrazine, semicarbazide, aminoguanidine, 2,2'-dipyridyl, o-phenanthroline, EDTA, diethyldithiocarbamate, KCN, dithiothreitol, 2-mercaptoethanol, Na2S2O4, and diisopropyl fluorophosphate, poor inhibition by Na2MoO4, ammonium persulfate, and NaN3 | Arthrobacter pascens | |
| N-ethylmaleimide | 48.1% inhibition at 1 mM | Arthrobacter pascens | |
| Ni2+ | 95.0% inhibition at 1 mM | Arthrobacter pascens | |
| Pb2+ | 11.9% inhibition at 1 mM | Arthrobacter pascens | |
| PMSF | 49.4% inhibition at 1 mM | Arthrobacter pascens | |
| Sn2+ | 41.7% inhibition at 1 mM | Arthrobacter pascens | |
| Zn2+ | 51.9% inhibition at 1 mM | Arthrobacter pascens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten-type kinetics | Arthrobacter pascens | |
| 0.95 | - |
cyclohexyl isocyanide | pH 7.5, 25°C | Arthrobacter pascens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
12 * 43000, SDS-PAGE | Arthrobacter pascens |
| 530000 | - |
gel filtration | Arthrobacter pascens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclohexyl isocyanide + H2O | Arthrobacter pascens | - |
N-cyclohexyl formamide | - |
? | |
| cyclohexyl isocyanide + H2O | Arthrobacter pascens F164 | - |
N-cyclohexyl formamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter pascens | E1CJF8 | - |
- |
| Arthrobacter pascens F164 | E1CJF8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 14.6fold by ammonium sulfate fractionation followed by two steps of hydrophobic interaction chromatography | Arthrobacter pascens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | the enzyme is expressed in cells growing on isonitrile as the sole carbon and nitrogen sources, highest enzyme activity is strain F164 in a nutrient medium containing N-benzylformamide. No induction by growth on glycerol and (NH4)2SO4 as the sole carbon and nitrogen sources, respectively | Arthrobacter pascens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.935 | - |
purified enzyme, substrate is cyclohexyl isocyanide, pH 7.5, 25°C | Arthrobacter pascens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pentyl isocyanide + H2O | - |
Arthrobacter pascens | N-1-pentylformamide | - |
? | |
| 1-pentyl isocyanide + H2O | - |
Arthrobacter pascens F164 | N-1-pentylformamide | - |
? | |
| benzyl isocyanide + H2O | - |
Arthrobacter pascens | N-benzylformamide | - |
? | |
| benzyl isocyanide + H2O | - |
Arthrobacter pascens F164 | N-benzylformamide | - |
? | |
| cyclohexyl isocyanide + H2O | - |
Arthrobacter pascens | N-cyclohexyl formamide | - |
? | |
| cyclohexyl isocyanide + H2O | the substrate is partly decomposed into N-cyclohexylformamide on spontaneous and chemical, nonenzymatic reaction under acidic conditions under pH 6.0, but is over pH 6.5 | Arthrobacter pascens | N-cyclohexyl formamide | - |
? | |
| cyclohexyl isocyanide + H2O | - |
Arthrobacter pascens F164 | N-cyclohexyl formamide | - |
? | |
| cyclohexyl isocyanide + H2O | the substrate is partly decomposed into N-cyclohexylformamide on spontaneous and chemical, nonenzymatic reaction under acidic conditions under pH 6.0, but is over pH 6.5 | Arthrobacter pascens F164 | N-cyclohexyl formamide | - |
? | |
| ethyl isocyanoacetate + H2O | - |
Arthrobacter pascens | N-formylglycine ethyl ester | - |
? | |
| methyl isocyanoacetate + H2O | - |
Arthrobacter pascens | N-formylglycine methyl ester | - |
? | |
| additional information | substrate specificity, isocyanomethyl phosphonic acid diethyl ether is a poor substrate giving isocyanomethyl phosphonic acid diethyl ether, overview | Arthrobacter pascens | ? | - |
? | |
| additional information | substrate specificity, isocyanomethyl phosphonic acid diethyl ether is a poor substrate giving isocyanomethyl phosphonic acid diethyl ether, overview | Arthrobacter pascens F164 | ? | - |
? | |
| tert-butyl isocyanide + H2O | - |
Arthrobacter pascens | N-tert-butylformamide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | 12 * 43000, SDS-PAGE | Arthrobacter pascens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| InhB | - |
Arthrobacter pascens |
| isonitrile hydratase | - |
Arthrobacter pascens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arthrobacter pascens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | most stable at 35°C, enzyme activity is rapidly lost above 40°C | Arthrobacter pascens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arthrobacter pascens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.7 | most stable in the range of pH 5.5-9.7 | Arthrobacter pascens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arthrobacter pascens | the enzyme is induced by N-benzylformamide and isonitriles benzyl isocyanide and cyclohexyl isocyanide, while benzylamine and N-benzylacetamide do not induce isonitrile hydratase activity | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | isonitrile hydratase is involved in the first step of isonitrile metabolism hydrating isonitrile to the corresponding N-substituted formamide | Arthrobacter pascens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
