Literature summary for 4.2.1.100 extracted from

  • Peters, F.; Shinoda, Y.; McInerney, M.J.; Boll, M.
    Cyclohexa-1,5-diene-1-carbonyl-coenzyme A (CoA) hydratases of Geobacter metallireducens and Syntrophus aciditrophicus: Evidence for a common benzoyl-CoA degradation pathway in facultative and strict anaerobes (2007), J. Bacteriol., 189, 1055-1060.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells Syntrophus aciditrophicus
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells Geobacter metallireducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
cyclohexa-1,5-diene-1-carbonyl-CoA pH 7.5, 30°C Syntrophus aciditrophicus
0.05
-
6-hydroxycyclohex-1-enecarbonyl-CoA
-
Geobacter metallireducens
0.085
-
cyclohexa-1,5-diene-1-carbonyl-CoA pH 7.5, 30°C Geobacter metallireducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
Ferguson blot analysis Geobacter metallireducens
92000
-
gel filtration Geobacter metallireducens
115000
-
gel filtration Syntrophus aciditrophicus
30000
-
2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis Geobacter metallireducens
30000
-
3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration Geobacter metallireducens
30000
-
4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration Syntrophus aciditrophicus
30400
-
2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis Geobacter metallireducens
30400
-
3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration Geobacter metallireducens
29900
-
4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration Syntrophus aciditrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O Syntrophus aciditrophicus involved in the benzoyl-CoA pathway 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O Desulfococcus multivorans involved in the benzoyl-CoA pathway 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O Geobacter metallireducens involved in the benzoyl-CoA pathway 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r

Organism

Organism UniProt Comment Textmining
Desulfococcus multivorans
-
strain DSMZ 2059, grown with benzoate as substrate
-
Geobacter metallireducens
-
strain DSMZ 7210, grown with benzoate as substrate
-
Syntrophus aciditrophicus
-
grown with benzoate as substrate
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Syntrophus aciditrophicus
recombinant protein using His-tag Geobacter metallireducens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
cell extract, pH 7.5, 30°C Geobacter metallireducens
1.8
-
cell extract, pH 7.5, 30°C Syntrophus aciditrophicus
2
-
cell extract, pH 7.5, 30°C Desulfococcus multivorans
730
-
purified enzyme, dehydration reaction, pH 7.5, 30°C Geobacter metallireducens
350
-
purified enzyme, hydration reaction, pH 7.5, 30°C Syntrophus aciditrophicus
550
-
purified enzyme, hydration reaction, pH 7.5, 30°C Geobacter metallireducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
-
Syntrophus aciditrophicus 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
-
Desulfococcus multivorans 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
-
Geobacter metallireducens 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O involved in the benzoyl-CoA pathway Syntrophus aciditrophicus 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O involved in the benzoyl-CoA pathway Desulfococcus multivorans 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O involved in the benzoyl-CoA pathway Geobacter metallireducens 6-hydroxycyclohex-1-enecarbonyl-CoA
-
r

Subunits

Subunits Comment Organism
trimer 3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration Geobacter metallireducens
dimer 2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis Geobacter metallireducens
tetramer 4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration Syntrophus aciditrophicus

Synonyms

Synonyms Comment Organism
dienoyl-CoA hydratase
-
Syntrophus aciditrophicus
dienoyl-CoA hydratase
-
Desulfococcus multivorans
dienoyl-CoA hydratase
-
Geobacter metallireducens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
175
-
cyclohexa-1,5-diene-1-carbonyl-CoA pH 7.5, 30°C Syntrophus aciditrophicus
280
-
cyclohexa-1,5-diene-1-carbonyl-CoA pH 7.5, 30°C Geobacter metallireducens