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Literature summary for 4.2.1.10 extracted from

  • Maneiro, M.; Peon, A.; Lence, E.; Otero, J.M.; Van Raaij, M.J.; Thompson, P.; Hawkins, A.R.; Gonzalez-Bello, C.
    Insights into substrate binding and catalysis in bacterial type I dehydroquinase (2014), Biochem. J., 462, 415-424 .
    View publication on PubMed
Search for more literature for 4.2.1.10

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure in complex with (2R)-2-methyl-3-dehydroquinic acid, a substrate analogue, at 1.5 A. Residues Gln236, Pro234 and Ala233 are located in the flexible substrate-covering loop. Gln236 is responsible for the folding of this loop and for the dramatic reduction of its flexibility, which triggers active site closure. Glu46 is important in bringing the substrate close to the lysine/histidine catalytic pocket to initiate catalysis Mycobacterium tuberculosis
crystal structure in complex with (2R)-2-methyl-3-dehydroquinic acid, a substrate analogue, at 1.5 A. Residues Gln236, Pro234 and Ala233 are located in the flexible substrate-covering loop. Gln236 is responsible for the folding of this loop and for the dramatic reduction of its flexibility, which triggers active site closure. Glu46 is important in bringing the substrate close to the lysine/histidine catalytic pocket to initiate catalysis Salmonella enterica subsp. enterica serovar Typhi

Inhibitors

Inhibitors Comment Organism Structure
(2R)-2-methyl-3-dehydroquinic acid compound is a reversible competive inhibitor of type 1 enzymes and a weak substrate of type 2 enzymes Salmonella enterica subsp. enterica serovar Typhi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Mycobacterium tuberculosis
0.015
-
 3-dehydroquinate pH 7.2, 25°C Mycobacterium tuberculosis
0.024
-
 3-dehydroquinate pH 7.2, 25°C Salmonella enterica subsp. enterica serovar Typhi

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Salmonella enterica subsp. enterica serovar Typhi P24670
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-2-methyl-3-dehydroquinic acid compound is a reversible competive inhibitor of type 1 enzymes and a weak substrate of type 2 enzymes Mycobacterium tuberculosis ?
-
 ?
3-dehydroquinate
-
 Mycobacterium tuberculosis 3-dehydroshikimate + H2O
-
 ?
3-dehydroquinate
-
 Salmonella enterica subsp. enterica serovar Typhi 3-dehydroshikimate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
aroD
-
 Mycobacterium tuberculosis
aroD
-
 Salmonella enterica subsp. enterica serovar Typhi
DHQ1
-
 Salmonella enterica subsp. enterica serovar Typhi
DHQ2
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.66
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Mycobacterium tuberculosis
1.1
-
 3-dehydroquinate pH 7.2, 25°C Salmonella enterica subsp. enterica serovar Typhi
154
-
 3-dehydroquinate pH 7.0, 25°C Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.305
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Salmonella enterica subsp. enterica serovar Typhi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
60
-
 (2R)-2-methyl-3-dehydroquinic acid pH 7.2, 25°C Mycobacterium tuberculosis
10270
-
 3-dehydroquinate pH 7.2, 25°C Mycobacterium tuberculosis