Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Nicotiana tabacum |
NtDHD/SHD-1 is expressed in Escherichia coli | Nicotiana tabacum |
NtDHD/SHD-2 | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | RNA interference of NtDHD/SHD-1 strongly inhibits DHD/SHD activity in transgenic tobacco plants | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | NtDHD/SHD-2 is the cytosolic isoenzyme | Nicotiana tabacum | 5829 | - |
cytosol | DHD/SHD-2 | Nicotiana tabacum | 5829 | - |
plastid | - |
Nicotiana tabacum | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dehydroquinate | Nicotiana tabacum | - |
dehydroshikimate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q6PUF9 | cv. Samsun NN | - |
Nicotiana tabacum | Q6PUG0 | cv. Samsun NN | - |
Nicotiana tabacum | Q6PUG0 | DHD/SHD-2; cytosolic isozyme | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dehydroquinate | - |
Nicotiana tabacum | dehydroshikimate + H2O | - |
r | |
additional information | the enzyme also shows shikimate dehydrogenase activity, EC 1.1.1.25 | Nicotiana tabacum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-dehydroquinate dehydratase /shikimate dehydrogenase isoform 1 | - |
Nicotiana tabacum |
3-dehydroquinate dehydratase /shikimate dehydrogenase isoform 2 | - |
Nicotiana tabacum |
3-dehydroquinate dehydratase/shikimate dehydrogenase | - |
Nicotiana tabacum |
DHD/SHD | - |
Nicotiana tabacum |
More | cf. EC 1.1.1.25 | Nicotiana tabacum |
NtDHD/SHD-1 | - |
Nicotiana tabacum |
NtDHD/SHD-2 | cytosolic isoenzyme | Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Nicotiana tabacum |
30 | 45 | - |
Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Nicotiana tabacum | |
NADH | - |
Nicotiana tabacum |