Cloned (Comment) | Organism |
---|---|
gene PERMA_1443, phylogenetic tree, recombinant expression of His-tagged enzyme lacking the signal peptide from codon-optimized carbonic anhydrase gene in Escherichia coli strain BL21(DE3), real-time PCR enzyme expression analysis | Persephonella marina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Persephonella marina | |
Al3+ | - |
Persephonella marina | |
Cu2+ | - |
Persephonella marina | |
Fe3+ | - |
Persephonella marina | |
Hg2+ | - |
Persephonella marina | |
Pb2+ | - |
Persephonella marina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.8 | - |
CO2 | pH 7.8, 25°C, recombinant enzyme | Persephonella marina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates the recombinant enzyme lacking the signal peptide | Persephonella marina | |
additional information | the recombinant enzyme activity is not affected significantly by the presence of Ca2+, Mg2+, or K+ ions | Persephonella marina | |
Zn2+ | required, metalloenzyme, activates the recombinant enzyme lacking the signal peptide | Persephonella marina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Persephonella marina | - |
CO2 + H2O | - |
r | |
H2CO3 | Persephonella marina DSM 14350 / EX-H1 | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Persephonella marina | C0QRB5 | - |
- |
Persephonella marina DSM 14350 / EX-H1 | C0QRB5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Persephonella marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Persephonella marina | H2CO3 | - |
r | |
CO2 + H2O | - |
Persephonella marina DSM 14350 / EX-H1 | H2CO3 | - |
r | |
H2CO3 | - |
Persephonella marina | CO2 + H2O | - |
r | |
H2CO3 | - |
Persephonella marina DSM 14350 / EX-H1 | CO2 + H2O | - |
r | |
additional information | enzyme activity measurement by determining the enzyme's CO2 hydration activity and esterase activity with substrate 4-nitrophenyl acetate. The recombinant enzyme lacking the signal peptide can efficiently catalyze the hydration of CO2, precipitating CaCO3 as calcite in the presence of Ca2+ | Persephonella marina | ? | - |
? | |
additional information | enzyme activity measurement by determining the enzyme's CO2 hydration activity and esterase activity with substrate 4-nitrophenyl acetate. The recombinant enzyme lacking the signal peptide can efficiently catalyze the hydration of CO2, precipitating CaCO3 as calcite in the presence of Ca2+ | Persephonella marina DSM 14350 / EX-H1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Persephonella marina |
Synonyms | Comment | Organism |
---|---|---|
alpha-type CA | - |
Persephonella marina |
PERMA_1443 | - |
Persephonella marina |
PMCA | - |
Persephonella marina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
4 | esterase assay at | Persephonella marina |
25 | - |
CO2hydration reaction, assay at | Persephonella marina |
35 | 45 | CA-catalyzed sequestration of CO2 hydration, assay at | Persephonella marina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | purified recombinant His-tagged enzyme lacking the signal peptide, pH 7.6, 15 min, slow decrease in the enzyme activity at temperatures up to 50°C and almost no effect beyond 50°C up to 100°C. The enzyme retains over 80% of its activity between 60°C and 100°C, the Tm is 84.5°C | Persephonella marina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
320000 | - |
CO2 | pH 7.8, 25°C, recombinant enzyme | Persephonella marina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
esterase assay at | Persephonella marina |
7.8 | 8.3 | assay at, the pH drops during the CO2 hydration reaction to pH 6.2 | Persephonella marina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, recombinant enzyme | Persephonella marina |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30000 | - |
CO2 | pH 7.8, 25°C, recombinant enzyme | Persephonella marina |