Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Atrazine | - |
Apis mellifera | |
Carbaryl | - |
Apis mellifera | |
carbofuran | - |
Apis mellifera | |
propoxur | - |
Apis mellifera | |
simazine | - |
Apis mellifera | |
tebuconazole | - |
Apis mellifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, metalloenzyme | Apis mellifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Apis mellifera | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 35fold from spermatheca by L-tyrosine-sulfanylamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3 | Apis mellifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spermatheca | - |
Apis mellifera | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
175 EU/mg protein activity in CO2 hydration reaction, purified native enzyme, pH and temperature not specified in the publication | Apis mellifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Apis mellifera | H2CO3 | - |
r | |
H2CO3 | - |
Apis mellifera | CO2 + H2O | - |
r | |
additional information | the enzyme is also active in an esterase assay with 4-nitrophenylacetate as substrate | Apis mellifera | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Apis mellifera |
Synonyms | Comment | Organism |
---|---|---|
Am CA | - |
Apis mellifera |
AmCA | - |
Apis mellifera |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
AmCA inhibition by the pesticides is also measured (IC50 values) using an esterase assay with 4-nitrophenylacetate as substrate | Apis mellifera | additional information | |
0.003 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | tebuconazole | |
0.0031 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | Carbaryl | |
0.0087 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | carbofuran | |
0.0165 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | Atrazine | |
0.0273 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | simazine | |
0.0321 | - |
measured with hydration of CO2 activity assay, pH and temperature not specified in the publication | Apis mellifera | propoxur |