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Literature summary for 4.2.1.1 extracted from

  • Perfetto, R.; Del Prete, S.; Vullo, D.; Carginale, V.; Sansone, G.; Barone, C.M.A.; Rossi, M.; Alasmary, F.A.S.; Osman, S.M.; AlOthman, Z.; Supuran, C.T.; Capasso, C.
    Cloning, expression and purification of the alpha-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis (2017), J. Enzyme Inhib. Med. Chem., 32, 1029-1035 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of soluble His-tagged enzyme in Escherichia coli strain ArcticExpress (DE3)RIL Mytilus galloprovincialis

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide the recombinant MgaCA shows a very similar acetazolamide inhibition features when compared to those of the native enzyme (MgaCA) Mytilus galloprovincialis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the recombinant MgaCA shows a very similar kinetic features when compared to those of the native enzyme (MgaCA) Mytilus galloprovincialis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the conserved three histidines, His94, His96, and His119 (hCA I numbering system) coordinate the Zn(II) ion that is crucial for catalysis Mytilus galloprovincialis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Mytilus galloprovincialis
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Mytilus galloprovincialis A0A0N9FGL8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mantle
-
 Mytilus galloprovincialis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
 Mytilus galloprovincialis CO2 + H2O
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 32000, recombinant enzyme, SDS-PAGE Mytilus galloprovincialis
monomer or dimer x * 50000, native enzyme, SDS-PAGE Mytilus galloprovincialis

Synonyms

Synonyms Comment Organism
alpha-CA
-
 Mytilus galloprovincialis
alpha-carbonic anhydrase
-
 Mytilus galloprovincialis
CAII
-
 Mytilus galloprovincialis
carbonic anhydrase II
-
 Mytilus galloprovincialis
MgaCA
-
 Mytilus galloprovincialis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Mytilus galloprovincialis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
410000
-
 CO2 CO2 hydration, pH 8.3, 22°C, native enzyme Mytilus galloprovincialis
420000
-
 CO2 CO2 hydration, pH 8.3, 22°C, recombinant enzyme Mytilus galloprovincialis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 assay at Mytilus galloprovincialis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000361
-
 acetazolamide CO2 hydration, pH 8.3, 22°C, recombinant enzyme Mytilus galloprovincialis
0.00038
-
 acetazolamide CO2 hydration, pH 8.3, 22°C, native enzyme Mytilus galloprovincialis

General Information

General Information Comment Organism
additional information the full nucleotide sequence encoding for the native MgaCA shows an open reading frame of 255 amino acid residues containing the conserved three histidines, His94, His96, and His119 (hCA I numbering system), which coordinate the Zn(II) ion crucial for catalysis, and the two gate-keeper residues, the Glu106 and Thr199. The mussel enzyme has a residue of lysine as substituent of the proton shuttle residue His64, explaining the relatively low catalytic activity of the native MgaCA, with the following kinetic parameters for the CO2 hydration reaction Mytilus galloprovincialis
physiological function in metazoans, the alpha-CA family is largely represented and plays a pivotal role in the deposition of calcium carbonate biominerals Mytilus galloprovincialis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
35000
-
 CO2 CO2 hydration, pH 8.3, 22°C, recombinant enzyme Mytilus galloprovincialis
36000
-
 CO2 CO2 hydration, pH 8.3, 22°C, native enzyme Mytilus galloprovincialis