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Literature summary for 4.2.1.1 extracted from

  • Kocyigit, U.M.; Dastan, S.D.; Taslimi, P.; Dastan, T.; Gulcin, I.
    Inhibitory effects of some drugs on carbonic anhydrase enzyme purified from Kangal Akkaraman sheep in Sivas, Turkey (2017), J. Biochem. Mol. Toxicol., 32, e22000 .
    View publication on PubMed
Search for more literature for 4.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
(1R,2S,3S,4S)-4,6-diamino-2-([3-O-[(1S,2S,3S,4S,5S)-2-amino-5-(aminomethyl)-3,4-dihydroxycyclohexyl]-alpha-L-arabinofuranosyl]oxy)-3-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-beta-L-glucopyranoside i.e. neosym Ovis aries
(2S,5R,6R)-3,3-dimethyl-7-oxo-6-(2-phenylacetamido)-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid i.e. penokain G Ovis aries
(7S)-7-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-3-[[(furan-2-carbonyl)sulfanyl]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid i.e. ceftiofur Ovis aries
Caffeine
-
 Ovis aries
dexamethasone
-
 Ovis aries
ivermectin
-
 Ovis aries
metamizole sodium
-
 Ovis aries
sulfamezathine
-
 Ovis aries
tavilin 50
-
 Ovis aries
tetramisol
-
 Ovis aries

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloenzyme Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Ovis aries
-
 CO2 + H2O
-
 r
H2CO3 Ovis aries Kangal Akkaraman
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-
Ovis aries Kangal Akkaraman
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzynme from erythrocytes, 243fold by L-tyrosine sulfanilamide affinity chromatography, to homogeneity Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 11626 EU/mg, purified enzyme, pH 7.4, 25°C, p-nitrophenylacetate substrate Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
 Ovis aries CO2 + H2O
-
 r
H2CO3
-
 Ovis aries Kangal Akkaraman CO2 + H2O
-
 r
additional information enzyme esterase activity assay using p-nitrophenylacetate as substrate Ovis aries ?
-
 ?
additional information enzyme esterase activity assay using p-nitrophenylacetate as substrate Ovis aries Kangal Akkaraman ?
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00038
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries dexamethasone
0.00276
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries ivermectin
0.00533
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries (7S)-7-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-3-[[(furan-2-carbonyl)sulfanyl]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
0.00824
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries Caffeine
0.0276
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries tavilin 50
0.0366
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries sulfamezathine
0.1147
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries tetramisol
0.2083
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries (1R,2S,3S,4S)-4,6-diamino-2-([3-O-[(1S,2S,3S,4S,5S)-2-amino-5-(aminomethyl)-3,4-dihydroxycyclohexyl]-alpha-L-arabinofuranosyl]oxy)-3-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-beta-L-glucopyranoside
0.2135
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries (2S,5R,6R)-3,3-dimethyl-7-oxo-6-(2-phenylacetamido)-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid
0.2855
-
 pH 7.4, 25°C, versus 4-nitrophenylacetate as substrate Ovis aries metamizole sodium