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Literature summary for 4.2.1.1 extracted from
- Temperature stability of poly-[hemoglobin-superoxide dismutase-catalase-carbonic anhydrase] in the form of a solution or in the lyophilized form during storage at -80°C, 4°C, 25°C and 37°C or pasteurization at 70°C (2016), Artif. Cells Nanomed. Biotechnol., 44, 41-47 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase, poly-[SFHb-SOD-CAT-CA], is a constructed type of blood substitute. The enzymes polyhemoglobin, superoxide dismutase, catalase, and carbonic anhydrase (SFHb, SOD, CAT, CA) are all cross-linked together to form a nano-sized soluble blood substitute. The enzymes activities of the freeze-dried cross-linked enzyme complex poly-[SFHb-SOD-CAT-CA] are more stable compared with the soluble poly-[SFHb-SOD-CAT-CA] and even more so when compared to the uncrosslinked solution of free SFHb, SOD, CAT and CA enzymes | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| the enzymes polyhemoglobin, superoxide dismutase, catalase, and carbonic anhydrase (SFHb, SOD, CAT, CA) are all cross-linked together to form a nano-sized soluble blood substitute. The enzymes activities of the freeze-dried cross-linked enzyme complex poly-[SFHb-SOD-CAT-CA] are more stable compared with the soluble poly-[SFHb-SOD-CAT-CA] and even more so when compared to the uncrosslinked solution of free SFHb, SOD, CAT and CA enzymes | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Homo sapiens | - |
CO2 + H2O | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | - |
Homo sapiens | CO2 + H2O | - |
r | |
| additional information | the carbonic anhydrase enzyme activity is measured by the pH change of 0.02 M Tris buffer. The reaction is initiated by the addition of substrate and the time needed for the pH of the reaction mixture to drop from pH 8.3 to pH 6.3 is recorded | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| poly-[Hb-SOD-CAT-CA] | - |
Homo sapiens |
| polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
at the pasteurization temperature of 70°C, the enzyme activity of carbonic anhydrase is 100% after 1 h, 97% after 2 h, and 94% after 3 h, freeze-dried enzyme. For the enzyme in solution, 87.6% activity remains after 30 min, 81.5% after 1 h, 62% after 2 h, and 50% activity remains after 3 h | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
assay at, starting pH value | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (poly-[Hb-SOD-CAT-CA]) contains all three major functions of red blood cells (RBCs) at an enhanced level. The crosslinked poly-[SFHb-SOD-CAT-CA] enzyme complex does not activate the complement pathway in rat plasma | Homo sapiens |
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