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Literature summary for 4.2.1.1 extracted from
- Expression and characterization of codon-optimized carbonic anhydrase from Dunaliella species for CO(2) sequestration application (2012), Appl. Biochem. Biotechnol., 167, 2341-2356.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Dunaliella |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| As3+ | inhibit activity | Dunaliella |  |
| Cd2+ | stimulate activity | Dunaliella | |
| CN- | inhibit activity | Dunaliella | |
| Co2+ | stimulate activity | Dunaliella | |
| F- | inhibit activity | Dunaliella | |
| Hg2+ | inhibit activity | Dunaliella | |
| NO2- | inhibit activity | Dunaliella | |
| Pb2+ | inhibit activity | Dunaliella | |
| SCN- | inhibit activity | Dunaliella | |
| Zn2+ | stimulate activity | Dunaliella | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
SDS-PAGE | Dunaliella |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dunaliella | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzmye shows esterase activity with 4-nitrophenyl phosphate | Dunaliella | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonic anhydrase | - |
Dunaliella |
| Dsp-aCAopt sp. | - |
Dunaliella |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Dunaliella |
| 65 | - |
only 34% of residual activity compared to 25°C | Dunaliella |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
half-denaturation temperature | Dunaliella |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 10 | 11 | more than 80% of enzymatic activity retained even after 24 h at 4°C | Dunaliella |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 10 | enzyme shows high stable activity at the two ranges which are around pH 7.6 and pH 10 | Dunaliella |
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