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Literature summary for 4.2.1.1 extracted from

  • Innocenti, A.; Zimmerman. S.; Ferry, J.G.; Scozzafava, A.; Supuran, C.T.
    Carbonic anhydrase inhibitors. Inhibition of the zinc and cobalt gamma-class enzyme from the archaeon Methanosarcina thermophila with anions (2004), Bioorg. Med. Chem. Lett., 14, 3327-3331.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
azide weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
Br-
-
Methanosarcina thermophila
Carbonate weak inhibitor of the Zn(II)-substituted enzyme, best inhibitor of the Co(II)-substituted enzyme Methanosarcina thermophila
CN- best anion inhibitors for Zn2+-substituted enzyme are cyanate and hydrogen sulfide Methanosarcina thermophila
CNO-
-
Methanosarcina thermophila
CO32-
-
Methanosarcina thermophila
Cyanate best inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
cyanide
-
Methanosarcina thermophila
HCO3-
-
Methanosarcina thermophila
HS- best anion inhibitors for Zn2+-substituted enzyme are cyanate and hydrogen sulfide Methanosarcina thermophila
HSO3-
-
Methanosarcina thermophila
hydrogen sulfide best inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
hydrogen sulfite weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
hydrogencarbonate good inhibitor of the Co(II)-substituted enzyme Methanosarcina thermophila
I-
-
Methanosarcina thermophila
additional information fluoride, chloride, and sulfate do not inhibit the Zn2+-substituted enzyme and the Co2+-substituted enzyme appreciably; Zn(II)- and Co(II)-substituted enzymes show a very different behavior against anion inhibitors.The best Zn-Cam anion inhibitors are hydrogen sulfide and cyanate whereas thiocyanate, azide, carbonate, nitrite, and hydrogensulfite are weaker inhibitors. The inhibition of the Zn(II)-substituted enzyme by sulfide is puzzling since Cam is proposed to be located outside the cytoplasmic membrane where it is exposed to high levels of sulfide in the anaerobic environments. The best inhibitors of the Co(II)-substituted enzyme is carbonate, followed by nitrate and hydrogencarbonate. Fluoride, chloride, and sulfate do not inhibit either Zn(II)- or Co(II)-substituted enzyme appreciably up to concentrations of 200 mM. This lack of inhibition may represent an adaptation to marine environments high in chloride and sulfate Methanosarcina thermophila
N3-
-
Methanosarcina thermophila
nitrate good inhibitor of the Co(II)-substituted enzyme Methanosarcina thermophila
nitrite weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila
NO2-
-
Methanosarcina thermophila
NO3-
-
Methanosarcina thermophila
SCN-
-
Methanosarcina thermophila
thiocyanate weak inhibitor of the Zn(II)-substituted enzyme Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the metal ion in the enzyme isolated directly from Methanosarcina thermophila is not been determined since only enzyme overproduced in Escherichia coli is characterized Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-
Methanosarcina thermophila P40881
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Methanosarcina thermophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.009
-
CO32- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
0.009
-
Carbonate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
0.05
-
HS- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
0.05
-
hydrogen sulfide Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
0.09
-
NO3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
0.09
-
CNO- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
0.09
-
nitrate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
0.09
-
Cyanate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
0.1
-
HCO3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
0.1
-
hydrogencarbonate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
0.68
-
CN- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
0.68
-
cyanide Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
1.8
-
HSO3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
1.8
-
hydrogen sulfite Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
2
-
HS- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
2
-
hydrogen sulfide Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.7
-
CNO- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.7
-
Cyanate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
4.9
-
N3- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
4.9
-
azide Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
5.3
-
I- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
5.3
-
I- Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
5.8
-
N3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
5.8
-
azide Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
6.1
-
SCN- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
6.1
-
thiocyanate Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
6.7
-
CO32- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
6.7
-
Carbonate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
6.8
-
NO2- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
6.8
-
nitrite Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
7
-
SCN- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
7
-
thiocyanate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
7.3
-
NO2- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
7.3
-
nitrite Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
11.7
-
HSO3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
11.7
-
hydrogen sulfite Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
22.2
-
Br- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
22.2
-
Br- Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
36.5
-
NO3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
36.5
-
nitrate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
42
-
HCO3- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
42
-
hydrogencarbonate Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
51.5
-
CN- 20°C, pH not specified in the publication, Co2+-substituted enzyme Methanosarcina thermophila
51.5
-
cyanide Co(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
160
-
Br- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
160
-
I- 20°C, pH not specified in the publication, Zn2+-substituted enzyme Methanosarcina thermophila
160
-
Br- Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila
160
-
I- Zn(II)-substituted enzyme, pH and temperature not specified in the publication Methanosarcina thermophila