Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | weak inhibitor of the Zn(II)-substituted enzyme | Methanosarcina thermophila | |
Br- | - |
Methanosarcina thermophila | |
Carbonate | weak inhibitor of the Zn(II)-substituted enzyme, best inhibitor of the Co(II)-substituted enzyme | Methanosarcina thermophila | |
CN- | best anion inhibitors for Zn2+-substituted enzyme are cyanate and hydrogen sulfide | Methanosarcina thermophila | |
CNO- | - |
Methanosarcina thermophila | |
CO32- | - |
Methanosarcina thermophila | |
Cyanate | best inhibitor of the Zn(II)-substituted enzyme | Methanosarcina thermophila | |
cyanide | - |
Methanosarcina thermophila | |
HCO3- | - |
Methanosarcina thermophila | |
HS- | best anion inhibitors for Zn2+-substituted enzyme are cyanate and hydrogen sulfide | Methanosarcina thermophila | |
HSO3- | - |
Methanosarcina thermophila | |
hydrogen sulfide | best inhibitor of the Zn(II)-substituted enzyme | Methanosarcina thermophila | |
hydrogen sulfite | weak inhibitor of the Zn(II)-substituted enzyme | Methanosarcina thermophila | |
hydrogencarbonate | good inhibitor of the Co(II)-substituted enzyme | Methanosarcina thermophila | |
I- | - |
Methanosarcina thermophila | |
additional information | fluoride, chloride, and sulfate do not inhibit the Zn2+-substituted enzyme and the Co2+-substituted enzyme appreciably; Zn(II)- and Co(II)-substituted enzymes show a very different behavior against anion inhibitors.The best Zn-Cam anion inhibitors are hydrogen sulfide and cyanate whereas thiocyanate, azide, carbonate, nitrite, and hydrogensulfite are weaker inhibitors. The inhibition of the Zn(II)-substituted enzyme by sulfide is puzzling since Cam is proposed to be located outside the cytoplasmic membrane where it is exposed to high levels of sulfide in the anaerobic environments. The best inhibitors of the Co(II)-substituted enzyme is carbonate, followed by nitrate and hydrogencarbonate. Fluoride, chloride, and sulfate do not inhibit either Zn(II)- or Co(II)-substituted enzyme appreciably up to concentrations of 200 mM. This lack of inhibition may represent an adaptation to marine environments high in chloride and sulfate | Methanosarcina thermophila | |
N3- | - |
Methanosarcina thermophila | |
nitrate | good inhibitor of the Co(II)-substituted enzyme | Methanosarcina thermophila | |
nitrite | weak inhibitor of the Zn(II)-substituted enzyme | Methanosarcina thermophila | |
NO2- | - |
Methanosarcina thermophila | |
NO3- | - |
Methanosarcina thermophila | |
SCN- | - |
Methanosarcina thermophila | |
thiocyanate | weak inhibitor of the Zn(II)-substituted enzyme | Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the metal ion in the enzyme isolated directly from Methanosarcina thermophila is not been determined since only enzyme overproduced in Escherichia coli is characterized | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Methanosarcina thermophila | P40881 | - |
- |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Methanosarcina thermophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
CO32- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
0.009 | - |
Carbonate | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
0.05 | - |
HS- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
0.05 | - |
hydrogen sulfide | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
0.09 | - |
NO3- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
0.09 | - |
CNO- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
0.09 | - |
nitrate | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
0.09 | - |
Cyanate | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
0.1 | - |
HCO3- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
0.1 | - |
hydrogencarbonate | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
0.68 | - |
CN- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
0.68 | - |
cyanide | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
1.8 | - |
HSO3- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
1.8 | - |
hydrogen sulfite | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
2 | - |
HS- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
2 | - |
hydrogen sulfide | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
4.7 | - |
CNO- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
4.7 | - |
Cyanate | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
4.9 | - |
N3- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
4.9 | - |
azide | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
5.3 | - |
I- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
5.3 | - |
I- | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
5.8 | - |
N3- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
5.8 | - |
azide | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
6.1 | - |
SCN- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
6.1 | - |
thiocyanate | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
6.7 | - |
CO32- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
6.7 | - |
Carbonate | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
6.8 | - |
NO2- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
6.8 | - |
nitrite | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
7 | - |
SCN- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
7 | - |
thiocyanate | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
7.3 | - |
NO2- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
7.3 | - |
nitrite | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
11.7 | - |
HSO3- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
11.7 | - |
hydrogen sulfite | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
22.2 | - |
Br- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
22.2 | - |
Br- | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
36.5 | - |
NO3- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
36.5 | - |
nitrate | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
42 | - |
HCO3- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
42 | - |
hydrogencarbonate | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
51.5 | - |
CN- | 20°C, pH not specified in the publication, Co2+-substituted enzyme | Methanosarcina thermophila | |
51.5 | - |
cyanide | Co(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
160 | - |
Br- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
160 | - |
I- | 20°C, pH not specified in the publication, Zn2+-substituted enzyme | Methanosarcina thermophila | |
160 | - |
Br- | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila | |
160 | - |
I- | Zn(II)-substituted enzyme, pH and temperature not specified in the publication | Methanosarcina thermophila |