Cloned (Comment) | Organism |
---|---|
genes cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6, real-time RT-PCR expression analysis, overview. Gene cah-3 is able to complement the function of Saccharomyces cerevisiae CA, NCE103, in vivo, expression in Saccharomyces cerevisiae wild-type strain CEN.PK2 and in mutant strain CEN.HE28-1A. Recombinant expression of His-tagged CAH-3, CAH-4a and CAH-5 in Escherichia coli strain BL21(DE3), but in vitro activity is only detectable for CAH-4a | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | RNAi by feeding is performed on wild-type worms for all genes, silencing of genes cah-3 and cah-4 reduces the life-span of the worms at 22°C, phenotypes, overview | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, isozymes CAH-3, CAH-4 and CAH-5 contain the three zinc-binding histidines that are important for enzyme activity, His94, His96, and His119, and also two ligands that provide a hydrogen bond to stabilize the zinc ion during catalysis, Gln-92 and Glu-117 | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Caenorhabditis elegans | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
six genes, cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6, possibly encoding alpha class carbonic anhydrases | - |
Purification (Comment) | Organism |
---|---|
recombinant isozymes CAH-3, CAH-4a and CAH-5 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | real-time RT-PCR analysis reveals the temporal expression pattern of genes cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6 | Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Caenorhabditis elegans | CO2 + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
alpha class carbonic anhydrase | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Caenorhabditis elegans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
685000 | - |
CO2 | purified recombinant CAH-4a, pH 7.5, 4°C | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Caenorhabditis elegans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
66.8 | - |
CO2 | purified recombinant CAH-4a, pH 7.5, 4°C | Caenorhabditis elegans |