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Literature summary for 4.2.1.1 extracted from

  • Ramanan, R.; Kannan, K.; Vinayagamoorthy, N.; Ramkumar, K.; Sivanesan, S.; Chakrabarti, T.
    Purification and characterization of a novel plant-type carbonic anhydrase from Bacillus subtilis (2009), Biotechnol. Bioprocess Eng., 14, 32-37.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide 62% residual activity at 1 mM Bacillus subtilis
Ca2+ 72% residual activity at 1 mM Bacillus subtilis
Cl- 10% residual activity at 1 mM Bacillus subtilis
EDTA complete inhibition at 1 mM Bacillus subtilis
HCO3- 7% residual activity at 1 mM Bacillus subtilis
Hg2+ complete inhibition at 1 mM Bacillus subtilis
Mn2+ 73% residual activity at 1 mM Bacillus subtilis
NO2- 41% residual activity at 1 mM Bacillus subtilis
NO3- 36% residual activity at 1 mM Bacillus subtilis
Pb2+ 33% residual activity at 1 mM Bacillus subtilis
sulfanilamide
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.09
-
4-nitrophenyl acetate in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 305% activity at 1 mM Bacillus subtilis
Cu2+ 165% activity at 1 mM Bacillus subtilis
Fe3+ 281% activity at 1 mM Bacillus subtilis
Mg2+ 107% activity at 1 mM Bacillus subtilis
SO42- 126% activity at 1 mM Bacillus subtilis
Zn2+ 473% activity at 1 mM Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis SA3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-75 gel filtration, DEAE cellulose column chromatography, and Sepharose-4B-L tyrosine sulfanilamide column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.62
-
crude extract, using 4-nitrophenyl acetate as substrate, at pH 8.3 and 37°C Bacillus subtilis
737.8
-
after 456.6fold purification, using 4-nitrophenyl acetate as substrate, at pH 8.3 and 37°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Bacillus subtilis 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Bacillus subtilis SA3 4-nitrophenol + acetate
-
?
CO2 + H2O
-
Bacillus subtilis H2CO3
-
r
CO2 + H2O
-
Bacillus subtilis SA3 H2CO3
-
r

Synonyms

Synonyms Comment Organism
carbonate hydro-lyase
-
Bacillus subtilis
carbonic anhydrase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the enzyme is not stable at temperatures of 60°C and above Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 13 activity is completely lost at a moderately acidic pH of 4.0 and a highly basic pH of 13.0 Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.099
-
sulfanilamide in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C Bacillus subtilis
0.22
-
acetazolamide in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis isoelectric focusing
-
6.1