Literature summary for 4.2.1.1 extracted from

Innocenti, A.; Hall, R.A.; Scozzafava, A.; Muehlschlegel, F.A.; Supuran, C.T.
Carbonic anhydrase activators: activation of the beta-carbonic anhydrases from the pathogenic fungi Candida albicans and Cryptococcus neoformans with amines and amino acids (2010), Bioorg. Med. Chem., 18, 1034-1037.

Activating Compound

Activating Compound	Comment	Organism
1-(2-aminoethyl)-piperazine	-	Cryptococcus neoformans
1-(2-aminoethyl)-piperazine	-	Candida albicans
2-(2-aminoethyl)pyridine	-	Cryptococcus neoformans
2-(2-aminoethyl)pyridine	-	Candida albicans
2-pyridyl-methylamine	-	Cryptococcus neoformans
2-pyridyl-methylamine	-	Candida albicans
4-(2-aminoethyl)-morpholine	-	Cryptococcus neoformans
4-(2-aminoethyl)-morpholine	-	Candida albicans
4-amino-L-phenylalanine	-	Cryptococcus neoformans
4-amino-L-phenylalanine	-	Candida albicans
D-Dopa	-	Cryptococcus neoformans
D-Dopa	best activator of CaNce103	Candida albicans
D-His	-	Cryptococcus neoformans
D-His	-	Candida albicans
D-Phe	micromolar affinity for Can2	Cryptococcus neoformans
D-Phe	micromolar affinity for CANce103	Candida albicans
D-Trp	-	Cryptococcus neoformans
D-Trp	-	Candida albicans
dopamine	-	Cryptococcus neoformans
dopamine	-	Candida albicans
histamine	-	Cryptococcus neoformans
histamine	-	Candida albicans
L-adrenaline	-	Cryptococcus neoformans
L-adrenaline	-	Candida albicans
L-Dopa	-	Cryptococcus neoformans
L-Dopa	best activator of CaNce103	Candida albicans
L-His	-	Cryptococcus neoformans
L-His	-	Candida albicans
L-Phe	-	Cryptococcus neoformans
L-Phe	-	Candida albicans
L-Trp	-	Cryptococcus neoformans
L-Trp	best Can2 activator	Cryptococcus neoformans
L-Trp	-	Candida albicans
L-Tyr	-	Cryptococcus neoformans
L-Tyr	-	Candida albicans
serotonin	-	Cryptococcus neoformans
serotonin	-	Candida albicans

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
8.2	-	CO2	CaNce103, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4	Candida albicans
9	-	CO2	Can2, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4	Cryptococcus neoformans

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism	UniProt	Comment	Textmining
Candida albicans	-	-	-
Cryptococcus neoformans	-	-	-

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Cryptococcus neoformans	H2CO3	-	r
CO2 + H2O	-	Candida albicans	H2CO3	-	r

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
390000	-	CO2	Can2, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4	Cryptococcus neoformans
680000	-	CO2	Can2, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4, in the presence of 0.01 mM histamine	Cryptococcus neoformans
800000	-	CO2	CaNce103, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4	Candida albicans
2010000	-	CO2	CaNce103, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4, in the presence of 0.01 mM histamine	Candida albicans