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Literature summary for 4.2.1.1 extracted from

  • Ynalvez, R.A.; Xiao, Y.; Ward, A.S.; Cunnusamy, K.; Moroney, J.V.
    Identification and characterization of two closely related beta-carbonic anhydrases from Chlamydomonas reinhardtii (2008), Physiol. Plant., 133, 15-26.
    View publication on PubMed
Search for more literature for 4.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol increases the activity of isozyme CAH7 by 69% but has no effect on the activity of isozyme CAH8 Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide
-
 Chlamydomonas reinhardtii
azide
-
 Chlamydomonas reinhardtii
cyanide
-
 Chlamydomonas reinhardtii
ethoxyzolamide
-
 Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Chlamydomonas reinhardtii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloenzyme Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, mature isozyme CAH8 Chlamydomonas reinhardtii
80000
-
 isoform CAH8, SDS-PAGE Chlamydomonas reinhardtii
84000
-
 isoform CAH7, SDS-PAGE Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii D66
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin chromatography Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the recombinant CAH7 protein has a specific activity of 3.1 Wilbur Anderson units/mg, while the recombinant CAH8 protein has an activity of 4.2 Wilbur Anderson units/mg Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Chlamydomonas reinhardtii H2CO3
-
 r
CO2 + H2O
-
 Chlamydomonas reinhardtii D66 H2CO3
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 40000, mature isozyme CAH8 Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
beta-carbonic anhydrase
-
 Chlamydomonas reinhardtii
CAH7 isoform Chlamydomonas reinhardtii
CAH8 isoform Chlamydomonas reinhardtii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.003
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii azide
0.0044
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii acetazolamide
0.015
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii cyanide
0.02
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii ethoxyzolamide
0.041
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii acetazolamide
0.044
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii ethoxyzolamide
0.061
-
 isozyme CAH7, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii cyanide
1.9
-
 isozyme CAH8, in 20 mM 4-(2-hydroxyethyl)-1-piperazine propane sulfonic acid, pH 8.0, at 4°C Chlamydomonas reinhardtii azide