Literature summary for 4.2.1.1 extracted from

Hilvo, M.; Baranauskiene, L.; Salzano, A.M.; Scaloni, A.; Matulis, D.; Innocenti, A.; Scozzafava, A.; Monti, S.M.; Di Fiore, A.; De Simone, G.; Lindfors, M.; Jaenis, J.; Valjakka, J.; Pastorekova, S.; Pastorek, J.; Kulomaa, M.S.; Nordlund, H.R.; Supuran, C.T.; Parkkila, S.
Biochemical characterization of CA IX, one of the most active carbonic anhydrase isozymes (2008), J. Biol. Chem., 283, 27799-27809.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli and in a baculovirus-Sf9 insect cell expression system, the recombinant proteins consist of either the CA IX catalytic domain only or the extracellular domain, which includes both the proteoglycan and catalytic domains, lacking the small transmembrane and intracytoplasmic regions of CA IX	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
acetazolamide	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.9	-	CO2	recombinant CA IX catalytic domain expressed in Escherichia coli	Homo sapiens
7	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the absence of Zn2+	Homo sapiens
7.3	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Co2+	Homo sapiens
7.3	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Mg2+	Homo sapiens
7.3	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Mn2+	Homo sapiens
7.3	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of Zn2+	Homo sapiens
7.5	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Zn2+	Homo sapiens
7.5	-	CO2	recombinant extracellular domain of CA IX expressed in Sf9 cells, in the absence of Zn2+	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	transmembrane protein	Homo sapiens	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	strongly activating effect, Co2+ induces an increase in the kcat/Km value	Homo sapiens
Mg2+	strongly activating effect, Mg2+ induces an increase in the kcat/Km value	Homo sapiens
Mn2+	strongly activating effect, Mn2+ induces an increase in the kcat/Km value	Homo sapiens
Zn2+	strongly activating effect, the recombinant extracellular domain of CA IX, which includes both the proteoglycan and catalytic domains, shows 2166% increase of activity in the presence of 0.05 mM Zn2+	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16790	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Homo sapiens	H2CO3	-	r

Synonyms

Synonyms	Comment	Organism
CA IX	-	Homo sapiens
carbonic anhydrase IX	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1100000	-	CO2	recombinant extracellular domain of CA IX expressed in Sf9 cells, in the absence of Zn2+	Homo sapiens
3800000	-	CO2	recombinant CA IX catalytic domain expressed in Escherichia coli	Homo sapiens
3800000	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the absence of Zn2+	Homo sapiens
4200000	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Zn2+	Homo sapiens
11000000	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Mg2+	Homo sapiens
12000000	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Co2+	Homo sapiens
25000000	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Mn2+	Homo sapiens
25000000	-	CO2	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of Zn2+	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000009	-	acetazolamide	recombinant CA IX catalytic domain expressed in Sf9 cells, in the presence of 0.05 mM Zn2+	Homo sapiens
0.000016	-	acetazolamide	recombinant extracellular domain of CA IX expressed in Sf9 cells, in the absence of Zn2+	Homo sapiens
0.000024	-	acetazolamide	recombinant CA IX catalytic domain expressed in Sf9 cells, in the absence of Zn2+	Homo sapiens
0.000025	-	acetazolamide	recombinant CA IX catalytic domain expressed in Escherichia coli	Homo sapiens
0.000041	-	acetazolamide	recombinant extracellular domain of CA IX expressed in Sf9 cells, in the presence of 0.05 mM Mn2+	Homo sapiens
0.000055	-	acetazolamide	recombinant extracellular domain of CA IX expressed in Sf9 cells, in the presence of 0.05 mM Mg2+	Homo sapiens
0.000056	-	acetazolamide	recombinant extracellular domain of CA IX expressed in Sf9 cells, in the presence of 0.05 mM Co2+	Homo sapiens
0.002325	-	acetazolamide	recombinant extracellular domain of CA IX expressed in Sf9 cells, in the presence of 0.05 mM Zn2+	Homo sapiens

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Release 2023.1 (January 2023)