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Literature summary for 4.2.1.1 extracted from
- Carbonic anhydrase: affinity purification and kinetic properties from rainbow trout lens (2006), Int. J. Appl. Chem., 2, 45-55.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sulfanilamide | noncompetitive | Oncorhynchus mykiss |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.1 | - |
4-nitrophenyl acetate | - |
Oncorhynchus mykiss | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Oncorhynchus mykiss |
| 27500 | - |
1 * 27500, SDS-PAGE | Oncorhynchus mykiss |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oncorhynchus mykiss | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oncorhynchus mykiss |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lens | - |
Oncorhynchus mykiss | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.7 | - |
- |
Oncorhynchus mykiss |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Oncorhynchus mykiss | 4-nitrophenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27500, SDS-PAGE | Oncorhynchus mykiss |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
- |
Oncorhynchus mykiss |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 30 | 15°C: about 35% of maximal activity, 30°C: about 80% of maximal activity | Oncorhynchus mykiss |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
0.025 M boric acid buffer | Oncorhynchus mykiss |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
maximal stability in 0.025 M Tris-SO4 buffer | Oncorhynchus mykiss |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000757 | - |
sulfanilamide | - |
Oncorhynchus mykiss | |
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Release 2023.1 (January 2023)
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