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Literature summary for 4.2.1.1 extracted from

  • Ulmasov, B.; Waheed, A.; Shah, G.N.; Grubb, J.H.; Sly, W.S.; Tu, C.; Silverman, D.N.
    Purification and kinetic analysis of recombinant CA XII, a membrane carbonic anhydrase overexpressed in certain cancers (2000), Proc. Natl. Acad. Sci. USA, 97, 14212-14217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.1

Application

Application Comment Organism
medicine overexpressed on cell surfaces of certain cancers, potential target for chemotherapeutic agents Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cDNA expressed in CHO-K1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H64A site-directed mutagenesis Homo sapiens
H64R site-directed mutagenesis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide
-
 Homo sapiens
ethoxzolamide
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane glycoprotein with an active extracellular CA domain Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Homo sapiens
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Homo sapiens 4-nitrophenol + acetate
-
 r
H2CO3
-
 Homo sapiens CO2 + H2O
-
 r

Synonyms

Synonyms Comment Organism
carbonic anhydrase XII
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0004
-
 CO2 pH 7.5, 25°C, wild-type CA XII Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 hydration of CO2 Homo sapiens
9.5
-
 hydrolysis of 4-nitrophenyl acetate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5 hydrolysis of 4-nitrophenyl acetate Homo sapiens
5.6 9.8 hydration of CO2 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000002
-
 ethoxzolamide pH 7.5, 25°C, wild-type CA XII Homo sapiens