Cloned (Comment) | Organism |
---|---|
wild-type and variants expressed in Escherichia coli BL21(DE3) or BL21(DE3) Gold | Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
R59A | site-directed mutagenesis | Methanosarcina thermophila |
R59C | site-directed mutagenesis | Methanosarcina thermophila |
R59E | site-directed mutagenesis | Methanosarcina thermophila |
R59H | site-directed mutagenesis | Methanosarcina thermophila |
R59K | site-directed mutagenesis | Methanosarcina thermophila |
R59M | site-directed mutagenesis | Methanosarcina thermophila |
R59Q | site-directed mutagenesis | Methanosarcina thermophila |
General Stability | Organism |
---|---|
freezing and thawing results in formation of aggregates, unfolded at 1.5 M guanidine hydrochloride | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.09 | - |
CO2 | pH 7.5, 37°C, variant R59H, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
6.5 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM ethylguanidine | Methanosarcina thermophila | |
9.45 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM aminoguanidine | Methanosarcina thermophila | |
11.7 | - |
CO2 | pH 7.5, 37°C, variant R59A, 150 mM guanidine | Methanosarcina thermophila | |
12.2 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM methylguanidine | Methanosarcina thermophila | |
12.8 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM 1.3-diaminoguanidine | Methanosarcina thermophila | |
13.6 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM guanidine | Methanosarcina thermophila | |
14.5 | - |
CO2 | pH 7.5, 37°C, variant R59A, 50 mM guanidine | Methanosarcina thermophila | |
15.7 | - |
CO2 | pH 7.5, 37°C, variant R59A | Methanosarcina thermophila | |
16.2 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM ethylguanidine | Methanosarcina thermophila | |
16.3 | - |
CO2 | pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
16.7 | - |
CO2 | pH 7.5, 37°C, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
17.1 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM guanidine | Methanosarcina thermophila | |
17.3 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM methylguanidine, variant R59Q, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
19.1 | - |
CO2 | pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
19.3 | - |
CO2 | pH 7.5, 37°C, variant R59E, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
21.8 | - |
CO2 | pH 7.5, 37°C, wild-type, without guanidine hydrochloride | Methanosarcina thermophila | |
25 | - |
CO2 | pH 7.5, 37°C, variant R59H, without guanidine hydrochloride | Methanosarcina thermophila | |
25.4 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM ethylamine | Methanosarcina thermophila | |
26.2 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM urea | Methanosarcina thermophila | |
33.4 | - |
CO2 | pH 7.5, 37°C, variant R59K, without guanidine hydrochloride | Methanosarcina thermophila | |
33.9 | - |
CO2 | pH 7.5, 37°C, variant R59Q, without guanidine hydrochloride | Methanosarcina thermophila | |
34 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM imidazole | Methanosarcina thermophila | |
34.2 | - |
CO2 | pH 7.5, 37°C, variant R59C | Methanosarcina thermophila | |
43.3 | - |
CO2 | pH 7.5, 37°C, variant R59M, without guanidine hydrochloride | Methanosarcina thermophila | |
47.6 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM aminoguanidine | Methanosarcina thermophila | |
51.4 | - |
CO2 | pH 7.5, 37°C, variant R59E, without guanidine hydrochloride | Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc enzyme | Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22873 | - |
3 * 22873, measuring A280 value of protein solution | Methanosarcina thermophila |
80000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Methanosarcina thermophila | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | P40881 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Methanosarcina thermophila | CO2 + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 22873, measuring A280 value of protein solution | Methanosarcina thermophila |
Synonyms | Comment | Organism |
---|---|---|
Cam | - |
Methanosarcina thermophila |
carbonic anhydrase | - |
Methanosarcina thermophila |
gamma-class carbonic anhydrase | - |
Methanosarcina thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | CO2 | pH 7.5, 37°C, variant R59A, 100 mM urea | Methanosarcina thermophila | |
10 | - |
CO2 | pH 7.5, 37°C, variant R59Q, without guanidine hydrochloride | Methanosarcina thermophila | |
12 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM 1.3-diaminoguanidine, variant R59H plus 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
13 | - |
CO2 | pH 7.5, 37°C, variant R59M, without guanidine hydrochloride | Methanosarcina thermophila | |
42 | - |
CO2 | pH 7.5, 37°C, variant R59H, without guanidine hydrochloride | Methanosarcina thermophila | |
67 | - |
CO2 | pH 7.5, 37°C, variant R59E, without guanidine hydrochloride | Methanosarcina thermophila | |
70 | - |
CO2 | pH 7.5, 37°C, variant R59A | Methanosarcina thermophila | |
110 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM imidazole | Methanosarcina thermophila | |
159 | - |
CO2 | pH 7.5, 37°C, variant R59M, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
160 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM ethylamine | Methanosarcina thermophila | |
310 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM ethylguanidine | Methanosarcina thermophila | |
320 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM aminoguanidine | Methanosarcina thermophila | |
350 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM aminoguanidine | Methanosarcina thermophila | |
520 | - |
CO2 | pH 7.5, 37°C, variant R59C | Methanosarcina thermophila | |
627 | - |
CO2 | pH 7.5, 37°C, variant R59Q, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
990 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM methylguanidine | Methanosarcina thermophila | |
2170 | - |
CO2 | pH 7.5, 37°C, variant R59C, 100 mM guanidine | Methanosarcina thermophila | |
2250 | - |
CO2 | pH 7.5, 37°C, variant R59A, 150 mM guanidine | Methanosarcina thermophila | |
2700 | - |
CO2 | pH 7.5, 37°C, variant R59A, 50 mM guanidine | Methanosarcina thermophila | |
2701 | - |
CO2 | pH 7.5, 37°C, variant R59A, 50 mM guanidine | Methanosarcina thermophila | |
2740 | - |
CO2 | pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
3270 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM guanidine | Methanosarcina thermophila | |
4720 | - |
CO2 | pH 7.5, 37°C, variant R59K, without guanidine hydrochloride | Methanosarcina thermophila | |
5690 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM methylguanidine | Methanosarcina thermophila | |
7140 | - |
CO2 | pH 7.5, 37°C, variant R59A, 100 mM ethylguanidine | Methanosarcina thermophila | |
20000 | - |
CO2 | pH 7.5, 37°C, variant R59E, without guanidine hydrochloride | Methanosarcina thermophila | |
22900 | - |
CO2 | pH 7.5, 37°C, wild-type, 100 mM guanidine hydrochloride | Methanosarcina thermophila | |
62900 | - |
CO2 | pH 7.5, 37°C, wild-type, without guanidine hydrochloride | Methanosarcina thermophila |