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Literature summary for 4.2.1.1 extracted from

  • Tripp, B.C.; Tu, C.; Ferry, J.G.
    Role of the arginine 59 in the gamma-class carbonic anhydrases (2002), Biochemistry, 41, 669-678.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and variants expressed in Escherichia coli BL21(DE3) or BL21(DE3) Gold Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
R59A site-directed mutagenesis Methanosarcina thermophila
R59C site-directed mutagenesis Methanosarcina thermophila
R59E site-directed mutagenesis Methanosarcina thermophila
R59H site-directed mutagenesis Methanosarcina thermophila
R59K site-directed mutagenesis Methanosarcina thermophila
R59M site-directed mutagenesis Methanosarcina thermophila
R59Q site-directed mutagenesis Methanosarcina thermophila

General Stability

General Stability Organism
freezing and thawing results in formation of aggregates, unfolded at 1.5 M guanidine hydrochloride Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.09
-
CO2 pH 7.5, 37°C, variant R59H, 100 mM guanidine hydrochloride Methanosarcina thermophila
6.5
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM ethylguanidine Methanosarcina thermophila
9.45
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM aminoguanidine Methanosarcina thermophila
11.7
-
CO2 pH 7.5, 37°C, variant R59A, 150 mM guanidine Methanosarcina thermophila
12.2
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM methylguanidine Methanosarcina thermophila
12.8
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM 1.3-diaminoguanidine Methanosarcina thermophila
13.6
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM guanidine Methanosarcina thermophila
14.5
-
CO2 pH 7.5, 37°C, variant R59A, 50 mM guanidine Methanosarcina thermophila
15.7
-
CO2 pH 7.5, 37°C, variant R59A Methanosarcina thermophila
16.2
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylguanidine Methanosarcina thermophila
16.3
-
CO2 pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride Methanosarcina thermophila
16.7
-
CO2 pH 7.5, 37°C, 100 mM guanidine hydrochloride Methanosarcina thermophila
17.1
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM guanidine Methanosarcina thermophila
17.3
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM methylguanidine, variant R59Q, 100 mM guanidine hydrochloride Methanosarcina thermophila
19.1
-
CO2 pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride Methanosarcina thermophila
19.3
-
CO2 pH 7.5, 37°C, variant R59E, 100 mM guanidine hydrochloride Methanosarcina thermophila
21.8
-
CO2 pH 7.5, 37°C, wild-type, without guanidine hydrochloride Methanosarcina thermophila
25
-
CO2 pH 7.5, 37°C, variant R59H, without guanidine hydrochloride Methanosarcina thermophila
25.4
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylamine Methanosarcina thermophila
26.2
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM urea Methanosarcina thermophila
33.4
-
CO2 pH 7.5, 37°C, variant R59K, without guanidine hydrochloride Methanosarcina thermophila
33.9
-
CO2 pH 7.5, 37°C, variant R59Q, without guanidine hydrochloride Methanosarcina thermophila
34
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM imidazole Methanosarcina thermophila
34.2
-
CO2 pH 7.5, 37°C, variant R59C Methanosarcina thermophila
43.3
-
CO2 pH 7.5, 37°C, variant R59M, without guanidine hydrochloride Methanosarcina thermophila
47.6
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM aminoguanidine Methanosarcina thermophila
51.4
-
CO2 pH 7.5, 37°C, variant R59E, without guanidine hydrochloride Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc enzyme Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22873
-
3 * 22873, measuring A280 value of protein solution Methanosarcina thermophila
80000
-
gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Methanosarcina thermophila
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila P40881
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Methanosarcina thermophila CO2 + H2O
-
r

Subunits

Subunits Comment Organism
trimer 3 * 22873, measuring A280 value of protein solution Methanosarcina thermophila

Synonyms

Synonyms Comment Organism
Cam
-
Methanosarcina thermophila
carbonic anhydrase
-
Methanosarcina thermophila
gamma-class carbonic anhydrase
-
Methanosarcina thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 CO2 pH 7.5, 37°C, variant R59A, 100 mM urea Methanosarcina thermophila
10
-
CO2 pH 7.5, 37°C, variant R59Q, without guanidine hydrochloride Methanosarcina thermophila
12
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM 1.3-diaminoguanidine, variant R59H plus 100 mM guanidine hydrochloride Methanosarcina thermophila
13
-
CO2 pH 7.5, 37°C, variant R59M, without guanidine hydrochloride Methanosarcina thermophila
42
-
CO2 pH 7.5, 37°C, variant R59H, without guanidine hydrochloride Methanosarcina thermophila
67
-
CO2 pH 7.5, 37°C, variant R59E, without guanidine hydrochloride Methanosarcina thermophila
70
-
CO2 pH 7.5, 37°C, variant R59A Methanosarcina thermophila
110
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM imidazole Methanosarcina thermophila
159
-
CO2 pH 7.5, 37°C, variant R59M, 100 mM guanidine hydrochloride Methanosarcina thermophila
160
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylamine Methanosarcina thermophila
310
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM ethylguanidine Methanosarcina thermophila
320
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM aminoguanidine Methanosarcina thermophila
350
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM aminoguanidine Methanosarcina thermophila
520
-
CO2 pH 7.5, 37°C, variant R59C Methanosarcina thermophila
627
-
CO2 pH 7.5, 37°C, variant R59Q, 100 mM guanidine hydrochloride Methanosarcina thermophila
990
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM methylguanidine Methanosarcina thermophila
2170
-
CO2 pH 7.5, 37°C, variant R59C, 100 mM guanidine Methanosarcina thermophila
2250
-
CO2 pH 7.5, 37°C, variant R59A, 150 mM guanidine Methanosarcina thermophila
2700
-
CO2 pH 7.5, 37°C, variant R59A, 50 mM guanidine Methanosarcina thermophila
2701
-
CO2 pH 7.5, 37°C, variant R59A, 50 mM guanidine Methanosarcina thermophila
2740
-
CO2 pH 7.5, 37°C, variant R59K, 100 mM guanidine hydrochloride Methanosarcina thermophila
3270
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM guanidine Methanosarcina thermophila
4720
-
CO2 pH 7.5, 37°C, variant R59K, without guanidine hydrochloride Methanosarcina thermophila
5690
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM methylguanidine Methanosarcina thermophila
7140
-
CO2 pH 7.5, 37°C, variant R59A, 100 mM ethylguanidine Methanosarcina thermophila
20000
-
CO2 pH 7.5, 37°C, variant R59E, without guanidine hydrochloride Methanosarcina thermophila
22900
-
CO2 pH 7.5, 37°C, wild-type, 100 mM guanidine hydrochloride Methanosarcina thermophila
62900
-
CO2 pH 7.5, 37°C, wild-type, without guanidine hydrochloride Methanosarcina thermophila