BRENDA - Enzyme Database
show all sequences of 4.1.99.5

Comparison of orthologous cyanobacterial aldehyde deformylating oxygenases in the production of volatile C3-C7 alkanes in engineered E. coli

Patrikainen, P.; Carbonell, V.; Thiel, K.; Aro, E.M.; Kallio, P.; Metab. Eng. Commun. 5, 9-18 (2017)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Prochlorococcus marinus), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Prochlorococcus marinus
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Synechococcus sp. RS9917
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Synechocystis sp. PCC 6803
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase, ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Nostoc punctiforme
Engineering
Protein Variants
Commentary
Organism
A134F
site-directed mutagenesis, the mutant shows slightly increased activity compared to wild-type
Prochlorococcus marinus
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Nostoc punctiforme ), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Nostoc punctiforme
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Prochlorococcus marinus, wild-type and mutant A134F), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Prochlorococcus marinus
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Synechococcus sp. RS9917
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Prochlorococcus marinus
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechocystis sp. PCC 6803
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechococcus sp. RS9917
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme ATCC 29133 / PCC 73102
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Prochlorococcus marinus MIT 9313
-
an alkane + formate + H2O + 2 NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nostoc punctiforme
B2J1M1
-
-
Nostoc punctiforme ATCC 29133 / PCC 73102
B2J1M1
-
-
Prochlorococcus marinus
Q7V6D4
-
-
Prochlorococcus marinus MIT 9313
Q7V6D4
-
-
Synechococcus sp. RS9917
A3Z5H6
-
-
Synechocystis sp. PCC 6803
Q55688
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
an alkane + formate + H2O + 2 NADP+
-
-
-
?
additional information
GC-MS analysis of the volatile alkane products
748564
Nostoc punctiforme
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Prochlorococcus marinus
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Synechocystis sp. PCC 6803
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Synechococcus sp. RS9917
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Prochlorococcus marinus MIT 9313
?
-
-
-
-
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ADO
-
Nostoc punctiforme
ADO
-
Prochlorococcus marinus
ADO
-
Synechococcus sp. RS9917
ADO
-
Synechocystis sp. PCC 6803
aldehyde deformylating oxygenase
-
Prochlorococcus marinus
aldehyde deformylating oxygenase
-
Synechococcus sp. RS9917
aldehyde deformylating oxygenase
-
Synechocystis sp. PCC 6803
aldehyde deformylating oxygenase
-
Nostoc punctiforme
cyanobacterial aldehyde deformylating oxygenase
-
Nostoc punctiforme
cyanobacterial aldehyde deformylating oxygenase
-
Prochlorococcus marinus
cyanobacterial aldehyde deformylating oxygenase
-
Synechococcus sp. RS9917
cyanobacterial aldehyde deformylating oxygenase
-
Synechocystis sp. PCC 6803
PMT_1231
-
Prochlorococcus marinus
RS9917_09941
-
Synechococcus sp. RS9917
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Prochlorococcus marinus
NADPH
-
Nostoc punctiforme
NADPH
-
Synechococcus sp. RS9917
NADPH
-
Synechocystis sp. PCC 6803
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Prochlorococcus marinus), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Prochlorococcus marinus
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Synechococcus sp. RS9917
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Synechocystis sp. PCC 6803
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase, ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
Nostoc punctiforme
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Prochlorococcus marinus
NADPH
-
Nostoc punctiforme
NADPH
-
Synechococcus sp. RS9917
NADPH
-
Synechocystis sp. PCC 6803
Engineering (protein specific)
Protein Variants
Commentary
Organism
A134F
site-directed mutagenesis, the mutant shows slightly increased activity compared to wild-type
Prochlorococcus marinus
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Nostoc punctiforme ), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Nostoc punctiforme
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Prochlorococcus marinus, wild-type and mutant A134F), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Prochlorococcus marinus
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Synechococcus sp. RS9917
additional information
installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Prochlorococcus marinus
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechocystis sp. PCC 6803
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechococcus sp. RS9917
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme ATCC 29133 / PCC 73102
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Prochlorococcus marinus MIT 9313
-
an alkane + formate + H2O + 2 NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
an alkane + formate + H2O + 2 NADP+
-
-
-
?
additional information
GC-MS analysis of the volatile alkane products
748564
Nostoc punctiforme
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Prochlorococcus marinus
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Synechocystis sp. PCC 6803
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Synechococcus sp. RS9917
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
?
-
-
-
-
additional information
GC-MS analysis of the volatile alkane products
748564
Prochlorococcus marinus MIT 9313
?
-
-
-
-
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
n-propane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Synechocystis sp. PCC 6803
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Synechococcus sp. RS9917
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Nostoc punctiforme ATCC 29133 / PCC 73102
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
748564
Prochlorococcus marinus MIT 9313
n-heptane + formate + H2O + 2 NADP+
-
-
-
?
Other publictions for EC 4.1.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747409
Wang
Identification of residues im ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2, Synechocystis sp. PCC 6803
BMC Biotechnol.
17
31-39
2017
-
-
-
-
21
-
-
8
-
2
-
3
-
9
-
-
-
-
-
-
-
-
11
1
7
-
-
-
28
-
-
-
1
-
-
-
-
-
-
1
-
21
-
-
-
-
8
-
2
-
3
-
-
-
-
-
-
-
-
11
1
-
-
-
28
-
-
-
-
-
2
2
-
9
9
748564
Patrikainen
Comparison of orthologous cya ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102, Prochlorococcus marinus, Prochlorococcus marinus MIT 9313, Synechococcus sp. RS9917, Synechocystis sp. PCC 6803
Metab. Eng. Commun.
5
9-18
2017
-
-
4
-
5
-
-
-
-
-
-
6
-
13
-
-
-
-
-
-
-
-
30
-
14
-
-
-
-
-
-
-
4
-
-
-
-
-
4
4
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746976
Park
Crystal structures of aldehyd ...
Limnothrix redekei, Limnothrix redekei KNUA012, Oscillatoria sp. KNUA011
Biochem. Biophys. Res. Commun.
477
395-400
2016
-
2
2
2
-
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
6
2
4
2
-
-
-
2
-
-
2
-
-
-
-
2
2
2
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
4
4
-
-
-
747351
Bao
Structure-oriented substrate ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2
Biotechnol. Biofuels
9
185
2016
-
-
1
-
13
-
-
7
-
-
-
2
-
6
-
-
1
-
-
-
-
-
14
-
6
1
-
-
8
1
-
-
1
-
-
-
-
-
1
1
-
13
-
-
-
-
7
-
-
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
8
1
-
-
-
-
2
2
-
8
8
747353
Zhang
Microbial synthesis of propan ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
Biotechnol. Biofuels
9
80
2016
-
-
1
-
3
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
3
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
747081
Warui
Efficient delivery of long-ch ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
Biochemistry
54
1006-1015
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748027
Rajakovich
Rapid reduction of the diferr ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
J. Am. Chem. Soc.
137
11695-11709
2015
-
-
-
-
-
-
-
-
-
1
-
2
-
8
-
-
-
1
-
-
-
-
10
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
748032
Shokri
Conversion of aldehyde to alk ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
J. Am. Chem. Soc.
137
7686-7691
2015
-
1
-
-
-
-
-
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
1
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749051
Hayashi
Role of cysteine residues in ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
PLoS ONE
10
e0122217
2015
-
-
1
-
8
-
-
1
-
1
-
2
-
7
-
-
1
-
-
-
-
-
10
-
2
1
-
5
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
10
-
1
-
5
-
-
-
-
-
-
3
3
-
-
-
749212
Jia
Structural insights into the ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2
Protein Cell
6
55-67
2015
-
-
1
1
3
-
-
-
-
1
-
2
-
6
-
-
1
1
-
-
-
-
6
1
5
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
726552
Das
Mechanistic insights from reac ...
Nostoc punctiforme
ACS Chem. Biol.
9
570-577
2014
-
-
1
-
-
-
1
-
-
1
-
1
-
2
-
-
1
1
-
-
-
-
6
-
3
1
-
-
3
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
6
-
1
-
-
3
1
-
-
-
-
1
1
-
-
-
746597
Buer
Insights into substrate and m ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Chem. Biol.
9
2584-2593
2014
-
-
-
1
1
-
1
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
-
4
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
747074
Waugh
Solvent isotope effects on al ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
Biochemistry
53
5537-5543
2014
-
-
1
-
-
-
-
1
-
1
-
2
1
3
-
-
1
1
-
-
-
-
6
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
1
-
2
1
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
726548
Aukema
Cyanobacterial aldehyde deform ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Catal.
3
2228-2238
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
8
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727215
Zhang
Conversion of fatty aldehydes ...
Synechococcus elongatus, Synechococcus elongatus PCC 7942
Biotechnol. Biofuels
6
86
2013
-
-
1
-
-
-
-
1
-
-
1
2
-
4
-
-
1
-
-
-
-
-
10
1
3
1
-
-
1
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
10
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
727312
Khara
Production of propane and othe ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
ChemBioChem
14
1204-1208
2013
-
-
1
-
4
-
-
-
-
1
-
2
-
3
-
-
1
1
-
-
-
-
8
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
727690
Pandelia
Substrate-triggered addition o ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
15801-15812
2013
1
-
1
-
-
-
-
1
-
1
-
1
-
2
-
-
1
1
-
-
-
-
2
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
727693
Paul
Probing the mechanism of cyano ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
5234-5237
2013
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727001
Li
Evidence for only oxygenative ...
Prochlorococcus marinus
Biochemistry
51
7908-7916
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
715293
Warui
Detection of formate, rather t ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
3316-3319
2011
-
-
1
-
-
-
-
-
-
1
-
2
-
4
-
-
1
1
-
-
-
-
4
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
715300
Li
Conversion of fatty aldehydes ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
6158-6161
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726957
Eser
Oxygen-independent alkane form ...
Nostoc punctiforme, Prochlorococcus marinus, Prochlorococcus marinus MIT9313, Synechococcus sp., Synechocystis sp.
Biochemistry
50
10743-10750
2011
-
-
4
-
-
-
4
-
-
-
-
-
-
8
-
-
-
4
-
-
-
-
15
-
8
4
-
-
-
4
-
-
4
-
-
-
-
-
4
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
4
-
-
-
4
-
-
-
-
4
4
-
-
-
713511
Schirmer
Microbial biosynthesis of alka ...
Nostoc punctiforme
Science
329
559-562
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
648395
Schneider-Belhaddad
Solubilization, partial purifi ...
Pisum sativum
Arch. Biochem. Biophys.
377
341-349
2000
4
-
-
-
-
-
7
-
2
1
1
1
-
4
1
-
1
-
-
1
1
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
7
-
-
2
1
1
1
-
1
-
1
-
1
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648396
Kobayashi
Cobalt proteins ...
Botryococcus braunii
Eur. J. Biochem.
261
1-9
1999
-
-
-
-
-
-
-
-
-
1
2
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648394
Dennis
A cobalt-porphyrin enzyme conv ...
Botryococcus braunii, Botryococcus braunii Austin
Proc. Natl. Acad. Sci. USA
89
5306-5310
1992
-
-
-
-
-
-
1
-
1
1
2
2
-
5
-
-
1
-
-
-
1
-
4
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
2
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648393
Dennis
Alkane biosynthesis by decarbo ...
Botryococcus braunii, Botryococcus braunii Austin
Arch. Biochem. Biophys.
287
268-275
1991
1
-
-
-
-
-
5
1
2
1
-
2
-
6
1
-
-
-
-
-
-
-
4
-
-
1
-
1
-
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
5
-
1
2
1
-
2
-
1
-
-
-
-
-
-
4
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
648392
Cheesbrough
Microsomal preparation from an ...
Podiceps nigricollis
J. Biol. Chem.
263
2738-2743
1988
2
-
-
-
-
-
9
1
2
1
-
1
-
3
1
-
-
-
-
1
-
-
2
-
-
1
-
1
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
9
-
1
2
1
-
1
-
1
-
-
-
1
-
-
2
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
648391
Cheesbrough
Alkane biosynthesis by decarbo ...
Pisum sativum
Proc. Natl. Acad. Sci. USA
81
6613-6617
1984
-
-
-
-
-
2
4
1
1
1
-
1
-
3
-
-
-
-
-
2
-
-
2
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
2
-
4
-
1
1
1
-
1
-
-
-
-
-
2
-
-
2
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-