BRENDA - Enzyme Database show
show all sequences of 4.1.99.5

Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

Wang, Q.; Bao, L.; Jia, C.; Li, M.; Li, J.J.; Lu, X.; BMC Biotechnol. 17, 31-39 (2017)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D143A
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
D49H
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
D49H
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
D49H/N123H
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
F150Y
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
L146T
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
L148R
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
additional information
cADO is engineered to improve specificity for short- to medium-chain aldehydes, site-directed mutagenesis of some residues in analogy to the more active enzyme from Prochlorococcus marinus strain MIT9313
Synechococcus elongatus PCC 7942
additional information
cADO is engineered to improve specificity for short- to medium-chain aldehydes, site-directed mutagenesis of some residues in analogy to the more active enzyme from Prochlorococcus marinus strain MIT9313
Synechocystis sp. PCC 6803
N123H
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
N123H
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
N149A
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Q110L
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Q49H/F150Y
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Q49H/N123H/F150Y
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
R62A
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
T146L
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
W178R
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Y122F
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Y150F
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
Y39F
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
n-Heptanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.3
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.32
-
n-Heptanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.33
-
n-Heptanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.34
-
n-Heptanal
recombinant mutant L146T, pH and temperature not specified in the publication; recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.35
-
n-Heptanal
recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.35
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.38
-
n-Heptanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
di-iron center
Synechococcus elongatus PCC 7942
Fe2+
di-iron center
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechocystis sp. PCC 6803
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechococcus elongatus PCC 7942
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechococcus elongatus PCC 7942 R2
-
an alkane + formate + H2O + 2 NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechococcus elongatus PCC 7942
Q54764
i.e. Synechococcus elongates strain PCC 7942
-
Synechococcus elongatus PCC 7942 R2
Q54764
i.e. Synechococcus elongates strain PCC 7942
-
Synechocystis sp. PCC 6803
Q55688
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
747409
Synechocystis sp. PCC 6803
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942 R2
an alkane + formate + H2O + 2 NADP+
-
-
-
?
additional information
cADO shows extreme low activity with kcat value below 1/min
747409
Synechococcus elongatus PCC 7942
?
-
-
-
-
additional information
cADO shows extreme low activity with kcat value below 1/min
747409
Synechococcus elongatus PCC 7942 R2
?
-
-
-
-
n-heptanal + O2 + 2 NADPH + 2 H+
-
747409
Synechocystis sp. PCC 6803
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-heptanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-heptanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942 R2
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
747409
Synechocystis sp. PCC 6803
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942 R2
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
enzyme structure comparisons, overview
Synechococcus elongatus PCC 7942
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0007
-
n-hexadecanal
recombinant mutant R62A, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0023
-
n-hexadecanal
recombinant mutant N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0025
-
n-hexadecanal
recombinant mutant Y122F, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0028
-
n-hexadecanal
recombinant mutant T146L, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0037
-
n-hexadecanal
recombinant mutant D49H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0038
-
n-hexadecanal
recombinant mutant Q110L, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0052
-
n-hexadecanal
recombinant mutant N149A, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.006
-
n-hexadecanal
recombinant mutant D143A, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0062
-
n-hexadecanal
recombinant mutant Y39F, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.007
-
n-hexadecanal
recombinant mutant Y150F, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0072
-
n-hexadecanal
recombinant mutant N123H, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0073
-
n-hexadecanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0075
-
n-hexadecanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.008
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0098
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.012
-
n-hexadecanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0122
-
n-hexadecanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0125
-
n-Heptanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0125
-
n-hexadecanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0127
-
n-hexadecanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0138
-
n-Heptanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0145
-
n-Heptanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0145
-
n-hexadecanal
recombinant mutant L146T, pH and temperature not specified in the publication; recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0152
-
n-Heptanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0157
-
n-Heptanal
recombinant mutant L146T, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0173
-
n-Heptanal
recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0245
-
n-hexadecanal
recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0302
-
n-Heptanal
recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Synechococcus elongatus PCC 7942
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Synechococcus elongatus PCC 7942
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D143A
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
D49H
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
D49H
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
D49H/N123H
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
F150Y
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
L146T
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
L148R
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
additional information
cADO is engineered to improve specificity for short- to medium-chain aldehydes, site-directed mutagenesis of some residues in analogy to the more active enzyme from Prochlorococcus marinus strain MIT9313
Synechococcus elongatus PCC 7942
additional information
cADO is engineered to improve specificity for short- to medium-chain aldehydes, site-directed mutagenesis of some residues in analogy to the more active enzyme from Prochlorococcus marinus strain MIT9313
Synechocystis sp. PCC 6803
N123H
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
N123H
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
N149A
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Q110L
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Q49H/F150Y
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Q49H/N123H/F150Y
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
R62A
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
T146L
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
W178R
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Y122F
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
Y150F
site-directed mutagenesis, altered activity compared to wild-type
Synechocystis sp. PCC 6803
Y39F
site-directed mutagenesis, altered activity compared to wild-type
Synechococcus elongatus PCC 7942
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
n-Heptanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.3
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.32
-
n-Heptanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.33
-
n-Heptanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.34
-
n-Heptanal
recombinant mutant L146T, pH and temperature not specified in the publication; recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.35
-
n-Heptanal
recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.35
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.38
-
n-Heptanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
di-iron center
Synechococcus elongatus PCC 7942
Fe2+
di-iron center
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechocystis sp. PCC 6803
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechococcus elongatus PCC 7942
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Synechococcus elongatus PCC 7942 R2
-
an alkane + formate + H2O + 2 NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
747409
Synechocystis sp. PCC 6803
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942 R2
an alkane + formate + H2O + 2 NADP+
-
-
-
?
additional information
cADO shows extreme low activity with kcat value below 1/min
747409
Synechococcus elongatus PCC 7942
?
-
-
-
-
additional information
cADO shows extreme low activity with kcat value below 1/min
747409
Synechococcus elongatus PCC 7942 R2
?
-
-
-
-
n-heptanal + O2 + 2 NADPH + 2 H+
-
747409
Synechocystis sp. PCC 6803
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-heptanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-heptanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942 R2
n-pentane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
747409
Synechocystis sp. PCC 6803
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
747409
Synechococcus elongatus PCC 7942 R2
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
enzyme structure comparisons, overview
Synechococcus elongatus PCC 7942
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0007
-
n-hexadecanal
recombinant mutant R62A, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0023
-
n-hexadecanal
recombinant mutant N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0025
-
n-hexadecanal
recombinant mutant Y122F, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0028
-
n-hexadecanal
recombinant mutant T146L, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0037
-
n-hexadecanal
recombinant mutant D49H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0038
-
n-hexadecanal
recombinant mutant Q110L, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0052
-
n-hexadecanal
recombinant mutant N149A, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.006
-
n-hexadecanal
recombinant mutant D143A, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0062
-
n-hexadecanal
recombinant mutant Y39F, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.007
-
n-hexadecanal
recombinant mutant Y150F, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0072
-
n-hexadecanal
recombinant mutant N123H, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0073
-
n-hexadecanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0075
-
n-hexadecanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.008
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0098
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.012
-
n-hexadecanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0122
-
n-hexadecanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0125
-
n-Heptanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0125
-
n-hexadecanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0127
-
n-hexadecanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0138
-
n-Heptanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0145
-
n-Heptanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0145
-
n-hexadecanal
recombinant mutant L146T, pH and temperature not specified in the publication; recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0152
-
n-Heptanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0157
-
n-Heptanal
recombinant mutant L146T, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0173
-
n-Heptanal
recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0245
-
n-hexadecanal
recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0302
-
n-Heptanal
recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
General Information
General Information
Commentary
Organism
additional information
residues close to the di-iron center (Tyr39, Gln110, Tyr122), the protein surface (Trp178), and involved in the hydrogen-bonding network (Arg62, Asp143) and the oligopeptide whose conformation changed (Leu/Thr146, Leu148, Asn149 and Tyr/Phe150) in the absence of the diiron center are identified. Comparison of the enzyme from Synechococcus elongates strain PCC 7942 and Synechocystis sp. PCC 6803, the first is more active than the latter against n-hexadecanal. Enzyme structure-function relationship analysis and comparisons, overview
Synechococcus elongatus PCC 7942
additional information
comparison of the enzyme from Synechococcus elongates strain PCC 7942 and Synechocystis sp. PCC 6803, the first is more active than the latter against n-hexadecanal. Enzyme structure-function relationship analysis and comparisons, overview
Synechocystis sp. PCC 6803
General Information (protein specific)
General Information
Commentary
Organism
additional information
residues close to the di-iron center (Tyr39, Gln110, Tyr122), the protein surface (Trp178), and involved in the hydrogen-bonding network (Arg62, Asp143) and the oligopeptide whose conformation changed (Leu/Thr146, Leu148, Asn149 and Tyr/Phe150) in the absence of the diiron center are identified. Comparison of the enzyme from Synechococcus elongates strain PCC 7942 and Synechocystis sp. PCC 6803, the first is more active than the latter against n-hexadecanal. Enzyme structure-function relationship analysis and comparisons, overview
Synechococcus elongatus PCC 7942
additional information
comparison of the enzyme from Synechococcus elongates strain PCC 7942 and Synechocystis sp. PCC 6803, the first is more active than the latter against n-hexadecanal. Enzyme structure-function relationship analysis and comparisons, overview
Synechocystis sp. PCC 6803
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0267
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0282
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0363
-
n-Heptanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.044
-
n-Heptanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.046
-
n-Heptanal
recombinant mutant L146T, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0473
-
n-Heptanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0495
-
n-Heptanal
recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0625
-
n-Heptanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0887
-
n-Heptanal
recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0267
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0282
-
n-Heptanal
recombinant wild-type enzyme, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0363
-
n-Heptanal
recombinant mutant D49H/N123H, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.044
-
n-Heptanal
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.046
-
n-Heptanal
recombinant mutant L146T, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0473
-
n-Heptanal
recombinant mutant L148R, pH and temperature not specified in the publication
Synechocystis sp. PCC 6803
0.0495
-
n-Heptanal
recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0625
-
n-Heptanal
recombinant mutant F150Y, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
0.0887
-
n-Heptanal
recombinant mutant W178R, pH and temperature not specified in the publication
Synechococcus elongatus PCC 7942
Other publictions for EC 4.1.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747409
Wang
Identification of residues im ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2, Synechocystis sp. PCC 6803
BMC Biotechnol.
17
31-39
2017
-
-
-
-
21
-
-
8
-
2
-
3
-
9
-
-
-
-
-
-
-
-
11
1
-
-
-
28
-
-
-
1
-
-
-
-
-
-
1
-
21
-
-
-
-
8
-
2
-
3
-
-
-
-
-
-
-
-
11
1
-
-
-
28
-
-
-
-
-
2
2
-
9
9
748564
Patrikainen
Comparison of orthologous cya ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102, Prochlorococcus marinus, Prochlorococcus marinus MIT 9313, Synechococcus sp. RS9917, Synechocystis sp. PCC 6803
Metab. Eng. Commun.
5
9-18
2017
-
-
4
-
5
-
-
-
-
-
-
6
-
13
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
4
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746976
Park
Crystal structures of aldehyd ...
Limnothrix redekei, Limnothrix redekei KNUA012, Oscillatoria sp. KNUA011
Biochem. Biophys. Res. Commun.
477
395-400
2016
-
2
2
2
-
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
6
2
2
-
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-
2
-
-
2
-
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2
2
2
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
4
4
-
-
-
747351
Bao
Structure-oriented substrate ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Biotechnol. Biofuels
9
185
2016
-
-
1
-
13
-
-
7
-
-
-
2
-
6
-
-
1
-
-
-
-
-
14
-
1
-
-
8
1
-
-
1
-
-
-
-
-
1
1
-
13
-
-
-
-
7
-
-
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
8
1
-
-
-
-
2
2
-
8
8
747353
Zhang
Microbial synthesis of propan ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
Biotechnol. Biofuels
9
80
2016
-
-
1
-
3
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
747081
Warui
Efficient delivery of long-ch ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
Biochemistry
54
1006-1015
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748027
Rajakovich
Rapid reduction of the diferr ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
J. Am. Chem. Soc.
137
11695-11709
2015
-
-
-
-
-
-
-
-
-
1
-
2
-
8
-
-
-
1
-
-
-
-
10
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
748032
Shokri
Conversion of aldehyde to alk ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
J. Am. Chem. Soc.
137
7686-7691
2015
-
1
-
-
-
-
-
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749051
Hayashi
Role of cysteine residues in ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
PLoS ONE
10
e0122217
2015
-
-
1
-
8
-
-
1
-
1
-
2
-
7
-
-
1
-
-
-
-
-
10
-
1
-
5
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
10
-
1
-
5
-
-
-
-
-
-
3
3
-
-
-
749212
Jia
Structural insights into the ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Protein Cell
6
55-67
2015
-
-
1
1
3
-
-
-
-
1
-
2
-
6
-
-
1
1
-
-
-
-
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
726552
Das
Mechanistic insights from reac ...
Nostoc punctiforme
ACS Chem. Biol.
9
570-577
2014
-
-
1
-
-
-
1
-
-
1
-
1
-
2
-
-
1
1
-
-
-
-
6
-
1
-
-
3
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
6
-
1
-
-
3
1
-
-
-
-
1
1
-
-
-
746597
Buer
Insights into substrate and m ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Chem. Biol.
9
2584-2593
2014
-
-
-
1
1
-
1
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
747074
Waugh
Solvent isotope effects on al ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
Biochemistry
53
5537-5543
2014
-
-
1
-
-
-
-
1
-
1
-
2
1
3
-
-
1
1
-
-
-
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
1
-
2
1
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
726548
Aukema
Cyanobacterial aldehyde deform ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Catal.
3
2228-2238
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
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8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727215
Zhang
Conversion of fatty aldehydes ...
Synechococcus elongatus
Biotechnol. Biofuels
6
86
2013
-
-
1
-
-
-
-
1
-
-
1
1
-
3
-
-
1
-
-
-
-
-
5
1
1
-
-
1
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
5
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
727312
Khara
Production of propane and othe ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
ChemBioChem
14
1204-1208
2013
-
-
1
-
4
-
-
-
-
1
-
2
-
3
-
-
1
1
-
-
-
-
8
-
1
-
-
-
1
-
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1
-
-
-
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-
1
1
-
4
-
-
-
-
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1
-
2
-
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-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
727690
Pandelia
Substrate-triggered addition o ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
15801-15812
2013
1
-
1
-
-
-
-
1
-
1
-
1
-
2
-
-
1
1
-
-
-
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2
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-
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1
-
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1
-
1
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1
-
1
-
1
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1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
727693
Paul
Probing the mechanism of cyano ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
5234-5237
2013
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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3
-
1
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-
-
1
-
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1
-
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-
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
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-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727001
Li
Evidence for only oxygenative ...
Prochlorococcus marinus
Biochemistry
51
7908-7916
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
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3
-
1
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1
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1
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-
-
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-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
715293
Warui
Detection of formate, rather t ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
3316-3319
2011
-
-
1
-
-
-
-
-
-
1
-
2
-
4
-
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1
1
-
-
-
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4
-
1
-
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1
-
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1
1
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-
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-
-
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1
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2
-
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1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
715300
Li
Conversion of fatty aldehydes ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
6158-6161
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
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1
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-
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1
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1
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726957
Eser
Oxygen-independent alkane form ...
Nostoc punctiforme, Prochlorococcus marinus, Prochlorococcus marinus MIT9313, Synechococcus sp., Synechocystis sp.
Biochemistry
50
10743-10750
2011
-
-
4
-
-
-
4
-
-
-
-
-
-
8
-
-
-
4
-
-
-
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15
-
4
-
-
-
4
-
-
4
-
-
-
-
-
4
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
4
-
-
-
4
-
-
-
-
4
4
-
-
-
713511
Schirmer
Microbial biosynthesis of alka ...
Nostoc punctiforme
Science
329
559-562
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
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-
-
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1
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